Among patients with unstable angina or myocardial infarction without ST-segment elevation, prasugrel did not significantly reduce the frequency of the primary end point, as compared with clopidogrel, and similar risks of bleeding were observed. (Funded by Eli Lilly and Daiichi Sankyo; TRILOGY ACS ClinicalTrials.gov number, NCT00699998.).
Mast cells cyclically synthesize and excrete a wide range of biogenesis products with different biological activities into the extracellular matrix and are regulators of local homeostasis both in normal conditions and in pathology – inflammation, oncogenesis, etc. The relative specificity of classical histochemical methods for detecting mast cells in relation to chromogenic to substrates causes certain difficulties in the selective study of the components of the secretome of mast cells, for example, heparin, histamine, chymase or tryptase. Therefore, immunomorphological techniques have become very popular, which identify specific substrates and allow differentiation of the components of the mast cell secretome. Mediators produced by mast cells promote neoangiogenesis, fibrillogenesis and re-epithelialization during the repair process.The aim of our work was to study the tryptase profile of the mast cell population of rat skin during the wound processusing an original combined method of immunohistochemical staining.Material and methods. The experiment involved 12 Wistar rats divided into two groups – intact (n=6) and with the existing wound process of the skin in the withers (n=6). The tryptase profile of mast cells was assessed on the 7th day of the wound process in comparison with the control group.Results. The results obtained showed a significant increase in the number of tryptase-positive mast cells on the 7th day of the wound process in the skin against the background of a general increase in the population of mast cells. Intragranular tryptase reserve was significantly increased. In contrast to the control, where mast cells with single tryptase-positive granules dominated, during the wound process, cells of this type were practically not detected in the skin (43.69±2.9% and 8.55±0.9%). The content of tryptase-positive mast cells with complete filling of the cytoplasm in the control group and the group of animals with a wound process was 14.24±1.2% and 38.03±2.9%, respectively.Conclusion. Thus, when modeling a wound, an increase in tryptase synthesis is detected both in individual MCs and within the entire MC population. This fact indicates that mast cell proteases can become a potential therapeutic target for improving wound regeneration by correcting immunogenesis, inflammation and fiber formation.
Целью настоящего исследования является изучение циклических изменений секреторных, деструктивных и пролиферативных процессов в коре надпочечных желез у норок. Материал и методы. Материал собран в период с декабря по апрель с месячным интервалом. Фрагменты надпочечных желез фиксировали в жидкостях Штиве, Буэна, Ценкера и в 10% растворе нейтрального формалина. Применяли ШИК-реакцию, окраску трихром-ШИК и тетрахром-ШИК. РНК выявляли реакцией Браше и галлоцианином по Эйнарсону. Материал, фиксированный в 10% растворе нейтрального формалина, использовали для изучения липидов. Результаты. Изучены циклические изменения морфофункциональных показателей коры надпочечника у норок. В декабре в коре надпочечных желез четко выражены клубочковая, пучковая и сетчатая зоны. В клубочковой зоне представлены полигональные клетки с умеренно вакуолизированной цитоплазмой, в пучковой зоне содержатся клетки, которые не отличаются от клеток клубочковой зоны. В январе в коре надпочечных желез обнаружено снижение функциональной активности корковой паренхимы и развитие обширных деструктивных изменений железистых клеток во всех зонах коркового вещества. В феврале отмечаются обширные пролиферативные процессы и повышение секреторной активности коры надпочечника. Мелкие клетки очагов пролиферации перемещаются в пучковую зону и превращаются в активно функционирующие клетки. В марте завершаются восстановительные процессы в коре надпочечника.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.