Objective: The goal of this study was paying attention on the chemical characterization of phytochemical compounds and their antioxidant activity of Sambucus nigra L. Methods: Phytochemical analysis was performed by Sambucus nigra L fruit extract. Total Phenol and Flavonoids content of elderberry fruit extract also determined using Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method. Antibacterial activity was performed by disk diffusion method and Antioxidant capacity was investigated by DPPH assay, butylhydroxytoluene used as a standard. Results: The richest anthocyanin in elderberry fruits was cyanidin-3-O-sambubioside. The antioxidant capacity obtained for elderberry extract proved that elderberry shown highest antioxidant activity, being the richest anthocyanins. The antioxidant capacity of elderberry fruit methanolic extract was recorded 62.56±1.12 percentages of scavenging activity. We also investigated antibacterial activity against four species Escherichia coli, Pseudomonas putida, Bacillus cereus, and Staphylococcus aureus. There E. coli was recorded 12.0 mm and Pseudomonas putida was recorded 0.34 mm zone of inhibition. Conclusion: The conclusion of our study is that Sambucus nisgra fruit extract has very high antioxidant activity which makes it recommendable for food industry and dietary supplement.
This study aim to investigate phytochemical and antioxidant activity of Rubus idaeus L fruit. The total phenolics, flavonoids and anthocyanins were evaluated. The antioxidant activities were investigated using three antioxidant assays 2,2diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing (FRAP) and 2,2′-azinobis(3-ethylbenzothiazoline-6sulfonic acid) (ABTS). The rasberry fruit contain of total phenolics (37.19±0.39 mg GAE/g), flavonoids (5.32±0.13 mg RU/ g) and total anthocyanin content (32.21±0.35 mg c-3-gE/g) were recorded. The antioxidant activity in DPPH assay IC 50 value 39.00±1.32 µg/ml, ABTS assay (0.85±0.02 mg AEAC/g) and FRAP assay 52.03±4.65 mM Fe2+/g were evaluated. These results presenting perspectives usage of R.idaeus fresh fruits was considerable levels of phytochemicals and antioxidant activity.
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