This communication gives the results of a further chemical study of the flavonoids of Lepidium perfoliatum L. (clasping pepperweed) [1]. By chromatography on Kapron using isopropanol and isobutanol eluents we isolated an individual flavonoid glycoside, which we have called "lepidoside." Lepidoside consists of small yellowish-green acieular crystals soluble in water and alcohols with mp 259-261 o C (from ethanol); [~]D-65° (methanol); Rf 0.68 (15% acetic acid), 0.79 (50% formic acid); kma x in ethanol, mp: 350,
The ethereal solution was separated into acidic, phenolic, and lactone fractions. The acidic fraction was chromatographe d on KSKsilica gel. On elntion with ether, acicular crystals with mp 201-202 ° C deposited which, on the basis of their IR spectrum, P,f value, and a mixed melting point were identified as the coumarin scopoletin. Then the unsaponifiable neutral fraction was chromatographed on alumina. From a methanolic eluate we isolated a substance with the composition C~sHs00, mp 139-140 ° C (from acetone), which gave the Liebermann-Burehard reaction for sterols. By comparing the IR spectra and ROt values and by means of a mixed melting point test the substance was identified as g-sitosterol. Seopoletin and 13-sitosterol have not previously been found in Artemisia dracunculus.
The epigeal part of Sedum caucasic~ (Grossh.) A. Bor. (Caucasian stonecrop), endemic to the Caucasus [i] was extracted with four portions of ethanol, and the extracts were worked up as described previously [2]. By two-dimensional paper chromatography, not fewer than twenty phenolic compounds, predominantly represented by flavonoids, were detected in the extract obtained. Chromatography of the ethyl acetate fractions on columns of Kapron [nylon-6] led to the isolation of four substances in the individual state (I-IV). When qualitative reactions and spectral studies in the UV region with ionizing and complex-forming reagents [3, 4] were performed, it was established that one of these substances isolated was a hydroxycoumarin (I) and three (II-IV) were flavonoids. p. 30.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.