The present investigation is the first attempt of its kind to analyze the phylogenetic association of Impatiens species of South India by placing them in the global Internal Transcribed Spacer (ITS) phylogeny of Impatiens. Representative samples from all the seven sections of genus Impatiens reported from South India were collected. Total genomic DNA was extracted from fresh plant leaf and Polymerise Chain Reaction (PCR) was carried out using ITS specific forward and reverse primers. Forty seven sequences from representatives of seven sections of Impatiens species from South India and 45 Impatiens sequences from other centers of Impatiens diversity were obtained from GenBank and used to reconstruct the evolutionary relationships within the genus Impatiens. Maximum Parsimony (MP) analysis was conducted by using PAUP*v.4.0b10. Bayesian Inference (BI) analysis was carried out in MrBayes v.3.2.2. Molecular phylogeny of South Indian Impatiens spp. based on ITS sequences showed the same association as that of morphological taxonomy. This study including all representative extant sections of South India, indicates that South India was colonized from Southeast Asia by two independent dispersal events i.e., once by ancestral species of sections Scapigerae, Epiphyticae, Tomentosae, Sub-Umbellatae and Racemosae and another time by ancestral species of sections Microsepalae and Annuae.
Only a few Impatiens spp. from South India (one of the five centers of diversity for Impatiens species) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiens species of South India, by placing them in the global phylogeny of Impatiens based on a combined analysis of two chloroplast genes. Thirty species of genus Impatiens were collected from different locations of South India. Total genomic DNA was extracted from fresh plant leaf, and polymerase chain reaction was carried out using atpB–rbcL and trnL-F intergenic spacer-specific forward and reverse primers. Thirteen sequences of Impatiens species from three centers of diversity were obtained from GenBank for reconstructing the evolutionary relationships within the genus Impatiens. Bayesian inference analysis was carried out in MrBayes v.3.2.2. This analysis supported Southeast Asia as the ancestral place of origin of extant Impatiens species. Molecular phylogeny of South Indian Impatiens spp. based on combined chloroplast sequences showed the same association as that of morphological taxonomy. Sections Scapigerae, Tomentosae, Sub-Umbellatae, and Racemosae showed Southeast Asian relationship, while sections Annuae and Microsepalae showed African affinity.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0574-8) contains supplementary material, which is available to authorized users.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.