Peanuts and peanut butter play an important role nutritionally in improving the diets of individuals in many parts of Africa, especially in the fight against child malnutrition. However, in developing countries such as Zimbabwe, most of the raw peanuts and peanut butter produced in backyard industries are sold in informal markets and rarely undergo formal safety inspection for aflatoxin contamination. The objective of the study was to determine the prevalence of aflatoxins in raw peanuts and backyard peanut butter sold at Mbare informal market. Ten (10) raw peanut samples and twenty (20) peanut butter samples were collected from Mbare informal market. Aflatoxin contamination was determined using liquid chromatography-mass spectrometry (LC-MS). The results revealed that sixty percent (60%) of the raw peanut samples were contaminated with total aflatoxin ranging from <0.75 to 426.4 μg/kg. One hundred percent (100%) of peanut butter samples were contaminated with total aflatoxins ranging from 4.7 μg/kg to 435.0 μg/kg. Aflatoxin B1 was the most prevalent aflatoxin in both raw peanuts (range, 1.2 μg/kg to 90.8 μg/kg) and peanut butter (range, 4.7 to 382.9 μg/kg). Forty percent (40%) of the raw peanuts and 95% of peanut butter samples exceeded the maximum limits of AFB1 as set by Zimbabwe legislation. The results suggest that raw peanuts and especially the peanut butter from backyard industries are heavily contaminated with aflatoxins and could constitute a possible health risk to consumers who regularly purchase these food commodities from informal markets.
The aim of this study was to investigate the effect of a finger millet-based broiler feed on the meat quality of chicken. Proximate nutrient composition was analyzed on the finger millet meal and a feed formulated with finger millet as the main energy source. The feed was formulated on percent crude protein (CP) basis using Pearson square method. An experiment was designed and conducted on Ross Hybrid chickens to determine the effect of the feed on lean tissue development. A two-tailed t-test statistical analysis was conducted at a significance level of 5% to determine the effect of the feed on fat deposition and lean tissue development in broiler muscle. The finger millet feed increased mean lean tissue mass by 3.47%. This study showed that use of finger millet feed reduced the fat deposition and favored protein deposition in broiler muscle (increased leanness). This study also showed that finger millet feed significantly enhanced broiler growth performance. We concluded that finger millet has the potential to replace maize meal in broiler feed formulation in order to produce lean and healthier meat for consumers.
BackgroundTriple class drug-resistant HIV-1 infection remains a global challenge in individuals with extensive antiretroviral treatment (ART) experience, in terms of high mortality and probability of onward transmission. New therapeutic options within old and new drug classes are therefore essential. We determined if patients failing salvage therapy in Botswana are eligible for maraviroc (MVC) and enfuvirtide (T20) viral entry inhibitors based on the coreceptor usage and drug-resistant mutations in envelope gp120 and gp41.MethodsA total of 38 deep salvage patients were included in the analysis. We amplified and sequenced gp41 and V3 regions of HIV-1 envelope. Drug resistance mutations were analysed according to the IAS-USA 2017 reference mutation lists. Coreceptor usage was determined using PSSM and Geno2Pheno using a false-positive rate (FPR) of 10%.ResultsAmong 38 participants, 34 (89%) were successfully sequenced and amplified gp41 and 26 (68%) gp120 V3 loop sequences were obtained. Major T20 mutation G36S was obtained in 1/34 samples (5.8%) within the study population. Polymorphisms I169V(97%), I135L(100%), E151A(70.6%) and N42S(70.6%) were detected in HR1 and HR2 of gp41. CXCR4 coreceptor associated use, mutation L34M in gp41 HR1 was detected in 2 samples (5%). Analysis of coreceptor usage showed (17/26) 65.4% use of CCR5, and a (9/26) 34.6% use of the CXCR4 coreceptor.ConclusionA moderately high proportion of treatment-experienced (deep salvage) participants had CXCR4 coreceptor using strains. The use of maraviroc in Botswana would require coreceptor tropism testing. Non-T20 treatment experience in Botswana reduces the prevalence of the major mutations that confer resistance to the drug. T20 is therefore a potential alternative drug for patients failing salvage therapy in Botswana.
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