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Background: According to modern concepts of burn disease development, one of the main burn complications is inflammation occurrence. Despite the large number of drugs used to treat the effects of chemical burns of the esophagus, we find conflicting information in the literature about their effectiveness. Moreover the problem of burn wound healing process quickening, as well as the prevention measures against possible post burn complications are really relevant if we aim to subjective portability of rehabilitation period facilitation. Methods: Study the immune system cytokine levels in immature rat blood serum under the simulation of 1 and 2 degree esophageal chemical burns in case of melanin administration as a drug. Was estimated ELISA using sets of reagents and instructions from Biotrack ELISA System company «Healthcare». Results: The significant increase of pro-inflammatory cytokines (PC) was shown mostly on day 7 of the experiment, which reflects the activity and severity of the disease process. In terms of further research the level of pro-inflammatory PC can be multidirectional. The significant decrease of anti-inflammatory cytokines amount was found under conditions of AEB at 15th and 21st days. At 1st and 2nd degree ABE the observed changes were multidirectional. Melanin administration resulted in the fast decrease of blood levels of all cytokines in our experiment to the values close to normal in conditions of esophagus chemical burn progress. Conclusion: Therefore, it was shown that some factors have the ability of changing the levels of pro inflammatory and anti-inflammatory cytokines in the models 1st and 2nd degree ABE and AEB in immature rats. The obtained data showed, that 1st degree ABE progressed without septic complications. In the models of 2nd degree ABE and AEB, the risk of poly organic failure remains. Melanin administration resulted in the fast decrease of blood levels of all cytokines in our experiment to the values close to normal in conditions of esophagus chemical burn progress.

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Background: Oxidative stress is the main cause of mortality in chemical burn-induced esophageal injury. Melanin, a natural antioxidant compound from yeast-like fungus Nadsoniella nigra strain X- 1, has been shown to decrease the content of lipid peroxidation products after burn. The aim of this study was to investigate the protective effect of melanin in the treatment of esophageal injury after a chemical burn. Methods: A alkali burn model was used to induce injury to the esophagus in immature rats. Changes in the levels of malondialdehyde, secondary products of lipid peroxidation (thiobarbituric acid reactive substances), superoxidase dismutase, and catalase in the blood, as well as changes in the esophagus tissue, were examined. Results: Melanin decreased the content of lipid peroxidation products following burn injury. Melanin increased the activity of superoxidase dismutase and reduced the activity of catalase, as well as reduced esophagus oxidative injury in our chemical burn model. Conclusion: Melanin treatment may protect against chemical burninduced esophageal injury, possibly by inhibiting burn-induced oxidative stress.

Introduction: As a result of alkaline substances entering the esophagus wall, necrosis occurs, as well as destruction of the mucous, submucosal and muscular layers of the esophagus. The cellular immune system plays an important role in regulating the various phases of the wound healing process, during which, depending on the time and through specific adhesion interactions, polymorphonuclear leukocytes, macrophages, and lymphocytes penetrate the site of the trauma and lead to an intensive proliferation of fibroblasts, endothelial cells, and keratinocytes. The substances of natural origins based on polyphenolic compounds are possible as a remedy for the normalization of physiological and biochemical parameters in a condition of various pathologies. Phaseolus vulgaris pods extract (PVPE) are an example of a rich source of bioactive compounds with proven human health benefits. Our results revealed imbalance of pro-inflammatory and anti-inflammatory cytokines in esophageal homogenates in rats with alkali burn of esophagus II degree, which can indicate a prolonged inflammation in the burned area. In the groups of animals with the alkaline burn of the esophagus, that were injected extract there can be traced a change of the indicators in the direction of normalization, indicating about the therapeutic effect of aqueous extract of pods of Phaseolus vulgaris. Objective: The cellular immune system plays an important role in regulating the various phases of the wound healing process, and can be used as a marker of pathological post-burn wound healing, Phaseolus vulgaris pods extract (PVPE) are an example of a rich source of bioactive compounds with proven human health benefits. The aim of the study was to investigate changes in cytokines levels in rat esophageal supernatants in the condition of alkali burn esophagus second degree (AEB 2) and treatment with Phaseolus vulgaris pods extract. Material and Methods: The animals were experimentally simulated with the alkali esophageal burn with 20% (grade II) solvent of NaOH. Levels of cytokines in esophageal tissue homogenates were done by ELISA. Results: In the present study, we investigate the imbalance of pro-inflammatory and anti-inflammatory cytokines in esophageal homogenates in rats with AEB 2, which can indicate a prolonged inflammation in the burned area. In the groups of animals with the AEB 2, that were injected extract there can be traced a change of the indicators in the direction of normalization. Conclusion: The administration of the extract, in conditions of alkaline burn of the esophagus, contributes change of the indicators in the direction of normalization indicating the therapeutic effect of aqueous extract of pods of Phaseolus vulgaris.