Public awareness and discussion about animal experiments and replacement methods has greatly increased in recent years. The term ‘the Three Rs’, which stands for the Replacement, Reduction and Refinement of animal experiments, is inseparably linked in this context. A common goal within the Three Rs scientific community is to develop predictive non-animal models and to better integrate all available data from in vitro, in silico and omics technologies into regulatory decision-making processes regarding, for example, the toxicity of chemicals, drugs or food ingredients. In addition, it is a general concern to implement (human) non-animal methods in basic research. Toward these efforts, there has been an ever-increasing number of Three Rs centres and platforms established over recent years — not only to develop novel methods, but also to disseminate knowledge and help to implement the Three Rs principles in policies and education. The adoption of Directive 2010/63/EU on the protection of animals used for scientific purposes gave a strong impetus to the creation of Three Rs initiatives, in the form of centres and platforms. As the first of a series of papers, this article gives an overview of the European Three Rs centres and platforms, and their historical development. The subsequent articles, to be published over the course of ATLA’s 50th Anniversary year, will summarise the current focus and tasks as well as the future and the plans of the Three Rs centres and platforms. The Three Rs centres and platforms are very important points of contact and play an immense role in their respective countries as ‘on the ground’ facilitators of Directive 2010/63/EU. They are also invaluable for the widespread dissemination of information and for promoting implementation of the Three Rs in general.

Мета роботи. Узагальнити сучасні літературні дані про токсичність сполук талію (Tl+), механізми їх токсичної дії та проаналізувати поширеність гострих отруєнь Tl+ за останні роки за кордоном і в Україні. Порівняти особливості клінічних проявів і наслідки інтоксикацій Tl+ з отриманою дозою і вмістом Tl+ в біосередовищах. Матеріал і методи. Проведено науковий аналіз даних літератури і власних спостережень щодо поширеності особливостей клінічних проявів і наслідків гострих отруєнь сполуками талію. Результати. Виявлено зростання поширеності поодиноких і групових випадків гострих отруєнь Tl+ в останні роки як за кордоном, так і в Україні. Встановлено, що серед клінічних проявів отруєнь Tl+ переважають неврологічні порушення, шлунково-кишкові та дерматологічні симптоми, вираженість яких переважно залежить від вмісту Tl+у біосередовищі. Висновки: Діагностика гострих отруєнь Tl+ у більшості випадків є запізнілою або посмертною. При поєднанні попередніх шлунково-кишкових і дерматологічних розладів з висхідними чутливими і руховими порушеннями іннервації в нижніх і верхніх кінцівках необхідно провести хіміко-токсикологічне визначення Tl+у біосередовищі хворого для виключення інтоксикації. Ключові слова: талій, механізми токсичної дії, гострі отруєння, діагностика.

The adoption of Directive 2010/63/EU on the protection of animals used for scientific purposes has given a major push to the formation of Three Rs initiatives in the form of centres and platforms. These centres and platforms are dedicated to the so-called Three Rs, which are the Replacement, Reduction and Refinement of animal use in experiments. ATLA’s 50th Anniversary year has seen the publication of two articles on European Three Rs centres and platforms. The first of these was about the progressive rise in their numbers and about their founding history; this second part focuses on their current status and activities. This article takes a closer look at their financial and organisational structures, describes their Three Rs focus and core activities (dissemination, education, implementation, scientific quality/translatability, ethics), and presents their areas of responsibility and projects in detail. This overview of the work and diverse structures of the Three Rs centres and platforms is not only intended to bring them closer to the reader, but also to provide role models and show examples of how such Three Rs centres and platforms could be made sustainable. The Three Rs centres and platforms are very important focal points and play an immense role as facilitators of Directive 2010/63/EU ‘on the ground’ in their respective countries. They are also invaluable for the wide dissemination of information and for promoting the implementation of the Three Rs in general.

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Objective is to summarise current literature data on the adverse effects of triclosan (TCS) biocide on human and animal health and potential risk for the development of endocrine-disruptor effects forjustification of the necessity to improve regulatory measures for its use in consumer products. Material and Methods. Analysis of literature data on the ways of influence of metabolic transformation in the body and toxicokinetics of TCS, its influence on human health and environment was performed. Targets and biomarkers of TCS exposure, its toxic properties and potential risk for the formation of endocrine-disruptor effects were described. Results. Necessity to optimise regulatory measures for the use of TCS in the personal hygienic means, general and curative beauty products, woven fabric for bedlinen and underwear, etc. has been justified. Key Words: triclosan biocide, toxic properties, endocrine-disruptor effects, regulatory measures.