In this study, a continuous cell line (named as CPB) was established from Siniperca chuatsi brain and has been subcultured >140 times. CPB cell line predominantly consisted of fibroblast-like cells that could grow better in Leibovitz's L-15 supplemented with 10% foetal bovine serum at 28° C. Polymerase chain reaction amplification of 18s recombinant (r)RNA confirmed the origin of this cell line from S. chuatsi. The CPB cell line was cryopreserved at different passage levels and revived successfully with 80-90% survival. The cell line was further characterized by chromosome number and transfection. The CPB cells were highly susceptible to infectious spleen and kidney necrosis virus (ISKNV) with a titre of 6·58-6·62 log TCID50 ml(-1) and numerous ISKNV particles were observed in the cytoplasm by transmission electron microscopy. At the same time, ISKNV infection was confirmed by reverse transcriptase polymerase chain reaction, immunodot blot and individual challenge experiments. The development and characterization of a new brain cell line from S. chuatsi were described in this study and it could be used as an in vitro tool for propagation of ISKNV and gene expression studies.
An 8-week feeding trial was conducted to evaluate effects of replacing fish meal (FM) with black soldier fly larvae meal (BSF) on growth performance, feed utilization, and plasma parameters, for juvenile yellow catfish, Pelteobagrus fulvidraco. Six isonitrogenous and isolipidic diets were prepared substituting the FM protein with BSF protein in the following amounts: 0 (control group), 10% (BSF10), 15% (BSF15), 20% (BSF20), 25% (BSF25) and 30% (BSF30). Three replicate groups of juvenile yellow catfish (initial weight of 1.20 ± 0.01 g) were stocked in circular tanks at a rate of 30 fish per tank. The results showed that 20% of the FM in the control diet could be replaced with BSF without significantly reducing weight gain, feed conversion ratio, or whole body and muscle proximate composition. Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipids, gross energy, or amino acids were not affected by 10% FM replacement. 30% FM replacement in the BSF30 diet significantly increased the concentration of cholesterol (CHO) and nitric oxide (NO) in the plasma, and significantly reduced the inhibition of superoxide radical anion formation. In conclusion, it appears that up to 20% of the FM in conventional yellow catfish diets can be replaced with BSF, and thus account for up to 8.9% of the total protein in the diet without causing a significant reduction in growth performance. The IJA appears exclusively as a peer-reviewed on-line open-access journal at http://www.siamb.org.il. To read papers free of charge, please register online at registration form. Sale of IJA papers is strictly forbidden.
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