The yeast-mycelial dimorphic fungus Aureobasidium pullulans was grown in chemostat culture in mineral medium under zinc-limiting and zinc-sufficient conditions. The Zn P uptake of both the yeast and mycelial growth form conformed to saturation kinetics and the Zn P uptake appeared to be energy dependent and no efflux was demonstrated. No significant difference between the K m and V mx values of the yeast and mycelial growth forms were found when grown under glucoselimiting (adequate zinc) conditions, with 7.6 WM Zn P in the feed medium. Growth conditions (zinc limitation or adequate zinc) were shown to influence the Zn P uptake capabilities as yeast cells harvested from zinc-limited chemostat culture had a higher affinity (K m = 0.13 WM) and a higher V mx (1.67 nmol Zn P min 3I (mg dry wt.) 3I ) than yeast cells harvested from zinc adequate chemostat culture (K m = 0.36 WM; V mx = 0.64 nmol Zn P min 3I (mg dry wt.) 3I ). z
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.