Aim The aim of this study was to screen potential plant growth promoting rhizobacterial (PGPR) actinobacterial isolate with effective inhibition against anthracnose causing fungal pathogen Colletotrichum capsici. Methods and Results In this study, actinobacterias were isolated from rhizosphere soil using dilution plate method and tested for antagonistic potential against pathogenic fungi C. capsici. In primary and secondary screening tests, the actinobacterial isolate BS‐26 displayed high antagonistic activity against the fungal pathogen. Isolate BS‐26 was identified as Streptomyces violaceoruber based on 16S rDNA sequencing. Furthermore, indole acetic acid production, phosphate solubilization and ammonia production have been confirmed in the S. violaceoruber that suggest their potential to be used as PGPR bacteria. A green house experiment showed that application of S. violaceoruber fermentation broth reduced the incidence of the chilli anthracnose and promoted the growth of chilli seedlings with a significant increase in germination %, total plant height, fresh weight and chlorophyll content when compared to controls. Conclusion Streptomyces violaceoruber can be applied as a biofertilizer and biocontrol agent for growing chillies against the attack of fungal pathogen C. capsici. Significance of Impact of the Study The damage caused by anthracnose disease is an issue of concern, affecting negatively the economy involved in chilli cultivation. As chemical methods of control have serious disadvantages, biocontrol approach using beneficial (PGPR) micro‐organisms shall be a better alternative to control crop diseases.
Objective: For control of microbial infections and diseases, various synthetic drugs and chemical formulations are currently in use. But due to the problem of microbial drug resistance, new alternative synthetic drugs have been explored. Similarly, antimicrobial activities of so many natural products have also been explored.Methods: In this various study extracts of cow dung possessed antimicrobial property against human pathogens like Klebsiella pneumonia and Escherichia coli.Results: The Indian cow dung extracted possessed superior antimicrobial activity than other cow dung types and showed antimicrobial property against all the test microorganisms. Since cow dung and buffalo dung are abundant in nature, which make the process cost effective with processing ease and thus are a promising solution for a variety of health problems in the near future.Conclusion: The medicinal properties of these cow dung and buffalo dung can be exploited to formulate drugs for several diseases caused by antibiotic resistant pathogenic microorganisms.
Objective: The present study deals with the isolation and identification of phytopathogenic fungi. The fungal isolates were Alternaria spp (Tomato early blight), Fusarium oxysporum (Fusarium wilt), Fusarium solani (daming off and root rot), Aspergillus flavus (Ear rot) and Collectotricumspp (Anthracnose).Methods: They were isolated from infected plant parts and were identified on the basis of colony morphology and Lactophenol cotton blue (LPCB) stains were used to identify microscopic examination of spore structures. Pure cultures of the isolates were subcultured and transferred onto differential media; potato dextrose agar, malt extract agar, czapek yeast extract agar and czapek dox agar for species identification using macromorphological characteristics The morphological characteristics of these fungal elements showed various kinds of spores have been identified up to genus/species level.Results: This study proves rapid and less expensive techniques to validate a primary alarm of contamination. Conclusion:The five fungus which was isolated from different plant parts were very effective in distruction of the plant and found that the production was reduced due to the infection. This rapid and less expensive technique to validate a primary alarm of contamination.
Aim The aim of present work was to explore the potential of Chlorella sp. SRD3 extracts for antioxidant and antibacterial activity along with the evaluation of minimum inhibitory concentration (MIC) and haemolytic activity to detect RBC cell damage. Methods and Results Screening and isolation of microalgae was performed using bold basal medium under normal illuminance (at 27°C) and microscopic observation. Growth of the microalgae was optimized using a different medium and light source. The isolated microalgae incubated under fluorescent light when cultured in F/2 medium showed a highest dry biomass yield of 3·77 ± 0·1 g l−1, when compared to the growth under direct sunlight (2·74 ± 0·07 g dwt l−1). The quantitative analysis of extracts revealed higher phenols, flavonoids and proanthocyanidins in ethyl acetate and hexane extracts followed by methanol. The antioxidant activity of extracts was tested against 1‐diphenyl‐2‐picrylhydrazyl and ABTS radical, its reducing power assay was performed. From antibacterial activity, the two extracts showed better inhibition against Gram‐negative bacteria. Also, they resulted in very low MIC values with effective activity against pathogens. In haemolytic activity, no haemolysis occurred, when the concentration (µg ml−1) was below 64 for methanol and 32 for ethyl acetate extract. In addition, Chlorella sp. extracts were characterized by GC‐MS analysis to detect the major compounds. Conclusion The polar extracts revealed satisfactory results against the clinical isolates and the compounds responsible were reflected in the GC‐MS spectrum. Significance and Impact of the Study The present study revealed significant biological potentials of the green alga, Chlorella sp. such as antioxidant, antibacterial and hemolytic activities. Therefore, this vital source might serve as a cost‐effective, alternative choice to the pharmaceutical and food industries in the near future.
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