Nowadays, many of the Agile based project development teams are distributed geographically across the globe. The teams are divided into onshore and offshore that work together to achieve on a common target. The teams in turn work in small iterations to minimize the effect of change in software requirements and at the same time developing regular communication between them. However different factors such as physical distance and lack of proper communication between the onshore and offshore teams become a hurdle between them leading to misunderstandings about software requirements. Though there are many advanced way of communication like video conferencing, voice chatting is available, it certainly has several disadvantages in getting the task completed. This paper gives an insight about these challenges faced in many of the software industries and it would allow different stakeholders within agile based onshore /offshore setting to better understand these challenges in eliciting their requirements.
Background: Decaprenylphosphoryl-β-D-ribose epimerase (DprE1), a flavoprotein enzyme engaged in the biosynthesis of decaprenylphosphoryl-β-D-arabinofuranose (DPA), is the only contributor of arabinose residues which is fundamental for the mycobacterium cell wall constituents. DprE1 is an interesting target for antitubercular agent and has been
exploring to develop potential chemical entities as antitubercular agents.Objective:The objective of study is the development of novel antitubercular agents targeting Mtb Decaprenylphosphoryl-βD-ribose epimerase (DprE1).Methods:A series of isoxazole encompassed 1, 2, 4-triazoles were designed based on the antitubercular potential of triazoles and structural features of DprE1 inhibitors. Designed 1, 2, 4-triazoles were synthesized and characterized by spectral
studies. The in vitro anti-TB activity of the compounds was screened against Mycobacterium tuberculosis H37Rv strain.by
Microplate Almar Blue Assay and in vitro cytotoxicity against normal cell lines by MTT assay. Molecular docking study
was carried out on DprE1 enzyme to understand designed compounds interactions with amino acid residues at the active
site.Results:Antitubercular activity data revels that eight compounds (6d, 6e,7d, 7e, 10d, 10e, 11d and 11e) have shown promising antitubercular activity with minimum inhibitory concentration at 1.6µg/mL. Cytotoxicity data of anti-TB active compounds demonstrate the good safety profile on normal cell lines.Conclusion:Eight compounds have shown promising antitubercular activity with good safety profile on normal cell lines.
Molecular docking study ascertain that the synthesized compounds have shown non-covalent interactions with amino acid
residues of DprE1 enzyme.
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Japanese encephalitis virus (JEV) is an arthropod-borne flavivirus belongs to the Flaviviridae family affecting millions of peoples worldwide.There is no specific drug approved for the treatment of this infection and also available vaccines are not effective against all the clinical isolates. Thus, the exploration of novel mechanistic pathways of existing molecules may help to develop more effective anti-JEV agents. Abscisic acid is a naturally occurring phytohormone released particularly in stress condition which controls leaf abscission. Recent studies have shown that the abscisic acid has the potential to inhibit virus by inhibiting protein disulfide isomerase enzyme which is important for the formation of viral proteins. Apart from this, abscisic acid could also reduce the neuroinflammation (a major hallmark of JEV infection) through stimulation of PPAR gamma. Thus, abscisic acid thereof could have the potential to develop as an anti-JEV agent.
Formerly with more augmented disabilities, Medical devices have become decisive device in many circumstances. As these are more perilous, the manufacturer should endow with an ideal medical device in aspects of safety & quality. To produce a homogeneous device globally, there should be some standards to be followed within an explicit country and standard throughout the globe, complying with the quality. In milieu of this resemblance of device globally, International Organization for Standard (ISO) has issued a standard, ISO 13485. This article is made to furnish the details about ISO 13485 and the Quality management system followed by United States manufacturer’s to market their devices within the country, i.e., 21 CFR Part 820.
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