No abstract

Thiamine (10(-4)-10(-6)M) inhibits lipid peroxidation in rat liver microsomes and free radical oxidation of oleic acid in vitro. Thiamine interacts with free radicals and hydroperoxides and is oxidized to thiochrome and thiamine disulfide. The antioxidant effect of thiamine is probably related to successive transfer of 2H(+)from the NH(2)group of the pyrimidine ring and H(+)from the thiazole ring (after its opening) to reactive substrates.

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On day 8 after ligation of the common bile duct in rats a significant increase in the serum content of total lipids, cholesterol, bilirubin and ALT, alkaline phosphatase, and gamma glutamyltransferase was observed. In the hepatic microsomal fraction there was a marked decrease in the content and activity of microsomal monooxygenases. Introperitoneal injections of berberine (10 mg/kg) for 6 days caused a partial normalization of hepatocyte plasma permeability and activity of microsomal flavin containing monooxyge nases. It is suggested that berberine is a substrate and inducer of flavin containing monooxygenases. The membrane stabilizing effect of berberine is probably realized at the level of inhibition of the prooxidant status of liver cells.

On the eighth day after ligation of the common bile duct in rats a significant increase in the serum content of total lipids, cholesterol bilirubin and ALT, alkaline phosphatase, and gamma-glutamyltransferase was observed. In the microsomal fraction there was a marked decrease in the content and activity of microsomal monooxygenases. Introperitoneal injection of berberine (10 mg/kg) for 6 days caused a partial normalization of permeability of hepatocytes plasma membranes and activity of microsomal flavin-containing monooxygenases. It is suggested that berberine is a substrate and inducer of flavin-containing monooxygenases. Membrane-stabilizing effect of berberine is probably realized at the level of inhibition of prooxidant status of liver cells.