SUMMARYIn patients with cutaneous T cell lymphomas such as mycosis fungoides B cells can frequently be detected in the lymphocytic dermal infiltrate. To analyse their immunoglobulin heavy chain gene repertoire, single B cells were obtained from tissue sections of two typical patients with mycosis fungoides using hydraulic micromanipulation followed by specific amplification of the respective gene segments by single-cell polymerase chain reaction (PCR) technique. A total of 21 V H DJ H genes was sequenced. From each individual B cell a single productive V H DJ H rearrangement was obtained. There was no clonal relationship detected between any of these rearrangements suggesting polyclonality of the infiltrating B cells. The representation of V H families was in accordance with the germ-line complexity. A remarkably high number of V H genes (5/13 in patient 1; 3/8 in patient 2) was completely or nearly germ-line-identical. Five of seven V H 4 family genes were nearly unmutated. On the other hand, most of the V H 3 gene family members were somatically mutated in an antigen-driven manner. The proportion of germ-line-identical V H genes, the usage of individual V H , D, J H gene elements, and the pattern of somatic mutations found in the B cells infiltrating skin lesions of patients with mycosis fungoides resembles the peripheral blood repertoire, suggesting a bystander role of these cells.
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