Ozone therapy combines the use of ozone gas and adjunct products called Reductant and the patient kit, which all contain fluoride. This study investigated the effect of between all the three regimes tested and the control with a trend favouring Ozone/Reductant/Patient Kit group. In our model, it appeared that Ozone treatment alone is not effective in protecting the enamel against demineralisation or promoting remineralisation, unless combined with the reductant/patient kit, which contain high levels of fluoride.