Identificación molecular de bacterias asociadas a la filosfera de plantas de arroz (Oryza sativa L), mediante técnicas de cultivo microbiano.Molecular identification of bacteria associated with the phyllosphere of rice plants (Oryza sativa L), through microbial culture techniques. ResumenLas plantas albergan una gran diversidad de microorganismos, como hongos, bacterias, etc., que interactúan con ella y tienen una funcionalidad que va desde la patogenicidad, hasta la protección de la misma. Se ha estudiado parte de esta diversidad microbiana a nivel de la filosfera en plantas de Oryza sativa (L) "arroz"; mediante microbiología molecular se ha caracterizado siete especies bacterianas entre las cuales sobresalieron especies Uncultured, es decir no cultivadas, además de Bacillus amyloliquefafaciens, Enterobacter asburiae, Klebsiella pneumoniae y Pantoea sp., todas ellas con un alto porcentaje de identidad; asimismo, mediante metagenómica dirigida se caracterizó trecientos ochenta y cinco especies bacterianas, de las que sobresalen pertenecen a los generos AbstractPlants are home to a great diversity of microorganisms; such as fungi, bacteria, etc., that interact with it and have a functionality ranging from the pathogenicity, to the protection of the same. Has been part of this microbial diversity at the level of phyllosphere in Oryza sativa (L) "rice plant"; using molecular microbiology has characterized seven bacterial species among which stood out, Uncultured species that is to say not cultivated, in addition of Bacillus amyloliquefafaciens, Enterobacter asburiae, Klebsiella pneumoniae and Pantoea sp., all them with a high percentage of identity; three hundred eighty five bacterial species was characterized through directed metagenomics, the species belonging to the genera Bacillus, Rhizobium, Pseudomonas, Mycobacterium, Nocardioides, Clostridium, Methylobac terium and Pantoea more they stand out. The families that stand out most are Entero bacteriaceae, Rhizobiaceae, Microbacteriaceae, Bacillaceae, Flavobacteriaceae, Pseudomonadaceae, Nocardiaceae, Mycobacteriaceae, Clostridiaceae and Methylobacteriaceae; we did not find Burkhol deria glumae.
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