No abstract

Adding small amounts of pig or buffalo body fat to cow or buffalo ghee results in the appearance of an extra peak located at high temperature in the melting and crystallization curves as determined by the differential scanning calorimetry (DSC) technique. Ghee adulterations with these animal fats at levels down to 5% are clearly seen in the crystallization diagrams. Quantitative measurements can be obtained by this method in the case of adulterations with buffalo body fat. On the other hand, this method does not detect vegetable oils such as coconut oil, and gives similar results for cottonseed‐fed buffalo ghee and ghee adulterated with animal body fats.

The differences in the melting diagrams and crystallization patterns of goat body fat and ghee, as determined by differential thermal analysis, provide a basis for the determination of adulteration in cow or buffalo ghee. The new endothermic peak on the melting diagram in samples having more than 10% goat body fat can be used for qualitative detection and the crystallization diagram can be used for quantitative estimation.

Citric acid whey was observed to be a suitable diluent for preserving buffalo semen both at room and at refrigerated temperature, as revealed by motility studies and conception rate. A 35 to 40% recovery rate was obtained with buffalo spermatozoa frozen in citric acid whey with glycerol.

An earlier report from this laboratory (Banerjee & Ganguli, 1971) was concerned with the electrophoretic and gel filtration behaviour of seminal plasma proteins in Zebu and buffalo bull semen. The chemical composition of Zebu semen has been reported by Mann (1964) and that of buffalo semen