Summary. Human erythrocyte ghosts where prepared by osmotic lysis and washed thoroughly with deionized distilled water. The resultant stroma were extracted twice with n‐butanol producing 81.8 % ± 2.7 solubilization of the membrane protein. The initial extract contained 5 % of the lipid present in intact ghosts. The second extract contained no detectable lipids. The solubilized glycoprotein possessed A, B and H blood group activity comparable to that of the intact ghosts at the same protein concentration. Fractionation studies suggested that the maximum serological activity was associated with a high molecular weight structure.
In contrast to most previous work demonstrating A, B and H blood group activities to be exhibited by erythrocyte glycolipids this study reports these blood group specificities to be present in human erythrocyte membrane glycoprotein.
General internal medicine instruction in an unsuccessful lecture-based course was replaced by a clinical problem-based 'junior clerkship'. Hospital and ambulatory-clinic instruction was centered around major common medical problems. Tutors willingly adopted a problem-based teaching approach and participated eagerly in a multi-component evaluation. Students and instructors responded favourably to questionnaires regarding the use of a problem list, the content and face validity of the course and the usefulness of the evaluations as learning experiences.
Abstract. Solubilized membrane glycoprotein derived from human erythrocytes by extraction in aqueous butanol lost all detectable capacity to bind γG (‘warm’) autoantibodies, whether the latter exhibited ‘Rh‐related’ or undefined serologic specificities. The same membrane glycoprotein, previously shown to retain A, B and H antigenic activities, also displayed complete loss of binding capacity for anti‐D (Rho), anti‐C (rh′), anti‐E (rh″), anti‐c (hr′) and anti‐e (hr″) isoantibodies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.