Increasing temperature and consequent changes in climate adversely affect plant growth and development, resulting in catastrophic loss of wheat productivity. For each degree rise in temperature, wheat production is estimated to reduce by 6%. A detailed overview of morpho-physiological responses of wheat to heat stress may help formulating appropriate strategies for heat-stressed wheat yield improvement. Additionally, searching for possible management strategies may increase productivity and sustainability of growing wheat. The major findings from this review are as follows: (1) heat stress significantly reduces seed germination and seedling growth, cell turgidity, and plant water-use efficiency; (2) at a cellular level, heat stress disturbs cellular functions through generating excessive reactive oxygen species, leading to oxidative stress; (3) the major responses of wheat to heat stress include the enhancement of leaf senescence, reduction of photosynthesis, deactivation of photosynthetic enzymes, and generation of oxidative damages to the chloroplasts; (4) heat stress also reduces grain number and size by affecting grain setting, assimilate translocation and duration and growth rate of grains; (5) effective approaches for managing heat stress in wheat include screening available germplasm under field trials and/or employing marker-assisted selection, application of exogenous protectants to seeds or plants, mapping quantitative trait locus conferring heat resistance and breeding; (6) a well-integrated genetic and agronomic management option may enhance wheat tolerance to heat. However, the success of applying various techniques of heat stress management requires greater understanding of heat tolerance features, molecular cloning, and characterization of genes. The overall success of the complex plant heat stress management depends on the concerted efforts of crop modelers, molecular biologists, and plant physiologists.

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Rice-based intensive cropping systems require high input levels making them less profitable and vulnerable to the reduced availability of labor and water in Asia. With continuous conventional puddled rice transplanting, the situation is exacerbated by damaged soil structure, declining underground water and decreasing land and water productivity. To minimize these negative effects a range of new crop establishment practices have been developed (zero tillage, dry direct seeding, wet direct seeding, water seeding, strip planting, bed planting, non-puddled transplanting of rice, mechanical transplanting of rice crop and combinations thereof) with varying effects on soil health, crop productivity, resource saving and global warming mitigation potential. Some of these allow Conservation Agriculture (CA) to be practiced in the rice-based mono-, double- and triple cropping systems. Innovations in machinery especially for smallholder farms have supported the adoption of the new establishment techniques. Non-puddling establishment of rice together with increased crop residue retention increased soil organic carbon by 79% and total N (TN) in soil by 62% relative to conventional puddling practice. Rice establishment methods (direct seeding of rice, system of rice intensification and non-puddled transplanting of rice) improve soil health by improving the physical (reduced bulk density, increased porosity, available water content), chemical (increased phosphorus, potassium and sulphur in their available forms) and biological properties (microbiome structure, microbial biomass C and N) of the soil. Even in the first year of its practice, the non-puddled transplanting method of rice establishment and CA practices for other crops increase the productivity of the rice-based cropping systems. Estimates suggest global warming potential (GWP) (the overall net effect) can be reduced by a quarter by replacing conventional puddling of rice by direct-seeded rice in the Indo-Gangetic Plains for the rice-based cropping system. Moreover, non-puddled transplanting of rice saves 35% of the net life cycle greenhouse gases (GHGs) compared with the conventional practice by a combination of decreasing greenhouse gases emissions from soil and increasing soil organic carbon (SOC). Though the system of rice intensification decreases net GHG emission, the practice releases 1.5 times greater N2O due to the increased soil aeration. There is no single rice establishment technology that is superior to others in all circumstances, rather a range of effective technologies that can be applied to different agro-climates, demography and farm typologies.

Background: Gamma-aminobutyric acid (GABA) is a non-protein amino acid present in all living things. GABA is mainly synthesized from glutamate by glutamate decarboxylase (GAD). In plants the enzymatic activity of GAD is activated by Ca 2+ /calmodulin binding (CaMBD) at the C-terminus in response to various stresses, allowing rapid GABA accumulation in cells. GABA plays a central role in not only stress responses but also many aspects of plant growth and development as a signaling molecules. Furthermore, it is known to be a health-promoting functional substance that exerts improvements in life-style related diseases such as hypertension, diabetes, hyperlipidemia, and so on. Previous reports indicated that CaMBD found plant GADs possess an autoinhibitory function because truncation of GAD resulted in extreme GABA accumulation in plant cells. Therefore, we attempted a genetic modification of rice GAD via genome editing technology to increase GABA levels in the edible part of rice.Results: In this study, we focused on GAD3, one of five GAD genes present in the rice genome, because GAD3 is the predominantly expressed in seeds, as reported previously. We confirmed that GAD3 has an authentic Ca 2+ / CaMBD that functions as an autoinhibitory domain. CRISPR/Cas9-mediated genome editing was performed to trim the coding region of CaMBD off from the OsGAD3 gene, then introducing this transgene into rice scutellum-derived calli using an all-in-one vector harboring guide RNAs and CRISPR/Cas9 via Agrobacterium to regenerate rice plants. Out of 24 transformed rice (T 1 ), a genome-edited rice line (#8_8) derived from two independent cleavages and ligations in the N-terminal position encoding OsGAD3-CaMBD and 40 bp downstream of the termination codon, respectively, displayed a AKNQDAAD peptide in the C-terminal region of the putative OsGAD3 in place of its intact CaMBD (bold indicates the trace of the N-terminal dipeptides of the authentic CaMBD). A very similar rice line (#8_ 1) carrying AKNRSSRRSGR in OsGAD3 was obtained from one base pair deletion in the N-terminal coding region of the CaMBD. Free amino acid analysis of the seeds (T 2 ) indicated that the former line contained seven-fold higher levels of GABA than wild-type, whereas the latter line had similar levels to the wild-type, although in vitro enzyme activities of recombinant GAD proteins based on the GAD3 amino acid sequence elucidated from these two lines in the absence of Ca 2+ /bovine CaM were both higher than wild-type counterpart. In addition to high level of GABA in #8_8, the average seed weight per grain and protein content were superior to wild-type and #8_1.(Continued on next page)

Thirty-six similar named aromatic rice landraces of Bangladesh were studied to assess the genetic variation for the agro-morphological traits through Mahalanobis D2 statistics. The landraces were grouped into four clusters, where no duplicate landrace was found. The inter-cluster distances were higher than intra-cluster distances indicating wider genetic diversity among the landraces of different clusters. The intra-cluster distances were lower in all the cases reflecting homogeneity of the landraces within the clusters. The principal component analysis showed that the first five components with vector values > 1 contributed 76.51% of the total variations. The highest number (13) of landraces was constellated in cluster III and the lowest (3) in cluster I. The intra- and inter-cluster distances were the maximum in cluster I (0.63) and between clusters I and IV (17.13), respectively and the minimum in cluster II (0.03) and between clusters III and IV (3.86), respectively. The maximum value of inter cluster distance indicated that the landraces belonging to cluster IV were far diverged from those of cluster I. So, it is expected in our results that parent’s selection for hybridization from the clusters IV and I may give the desirable heterosis for heterotic rice hybrids. Besides, the cluster mean revealed that the crosses between the genotypes of cluster I with those of clusters IV would exhibit high heterosis for maximum good characters. Hence, yield, grain breadth, days to maturity, culm diameter, ligule length had maximum contribution towards genetic divergence. Besides, evaluation of agro-morphological characters demonstrated that the aromatic rice landraces under the present study possessed a considerable genetic diversity. Therefore, similar and duplicate named aromatic rice landraces need to be conserved in Genebank for future breeding programme. Bangladesh Rice j. 2018, 22(1): 45-56