The study of the influence of selenium on the regeneration and fecundity of the crab Sesarma boulengeri Calman has been conducted. The results demonstrated: The effect of selenium on the regeneration of walking legs of S. boulengeri Calman. Morphology of the regenerated appendage was different from control specimen. The speed of growth in the regenerated bud was lower than the control and it was depended on the size class of the crab. Regeneration index (R) was higher in the small crabs than in the large ones and in single autotomy than in double autotomy. Fecundity varied, when females exposed to selenium (0.27, 1.35, 2.7 ppm). It showed a decrease with all stages, especially in the first and second stage. The eggs in the last stage suffered more teratogens with exposure time and the concentration of selenium. 3

To assess and compare clinical, hormonal, and metabolic factors with blood levels of RBP4, PAI-1, and trace elements in women with and without polycystic ovarian syndrome (PCOS). A cross-sectional clinical investigation was undertaken. From December 2020 until January 2022, samples were taken at the Basrah Hospital for Obstetrics and Children's infertility center. Significant changes (p< 0.05) were in HOMA-IR, E2 and Ts. Levels of PAI-1, RBP4, AMH, LH, LH/FSH, PRL and Cu were significantly (p<0.01) increased, and levels of Se, Zn, Mg and E2/T were significantly (p<0.01) decreased, between the patient (1o PCOS and 2o PCOS) and control groups, the QUICKI level did not differ significantly (p>0.05). Compared to the control group, FSH levels were especially (p<0.05) higher in non-obese PCOS patients and lower in obese PCOS patients. Area under the receiver operating characteristics (ROC) curve (AUC) results indicate RBP4 and PAI-1 may be more effective predictors biomarkers for PCOS in expectant women. While trace elements might be considered a protective factor in the emergence of PCOS, metabolic abnormalities and IR in PCOS-affected individuals are associated with the levels of RBP4 and PAI-1, which appear to be a more acceptable diagnostic marker in the early prediction of PCOS. Keywords: Polycystic Ovary Syndrome, RBP4, PAI-1, Trace elements.

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