Soybean is a major source of protein meal in the world. Soybean kunitz trypsin inhibitor (SKTI) protein is a responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The primary objective of this research was to identify DNA markers linked to the Ti locus controlling presence and absence of kunitz trypsin inhibitor protein. Two mapping populations were developed. Population 1 was derived from a cross between cultivar Jinpumkong2 (TiTi) and C242 (titi). Population 2 was made from a mating between cultivar Clark (TiTi) and C242. The F 1 plants were grown in the greenhouse to produce F 2 seeds. Each F 2 seed from F 1 plants was analyzed electrophoretically to determine the presence of the SKTI protein band. One-thousand RAPD primers, 342 AFLP primer sets, and 35 SSR primers were used to map Ti locus in population 1 and 2. The presence of SKTI protein was dominant to the lack of a SKTI protein and kunitz trypsin inhibit protein band was controlled by a single locus. Twelve DNA markers (4 RAPD, 4 AFLP, and 3 SSR) and Ti locus were found to be genetically linked in population 1 consisted with 94 F 2 individual plants. Three SSR markers (Satt409, Satt228, and Satt429) were linked with Ti locus within 10 cM. Satt228 marker was tightly linked with Ti locus. Satt228 marker was tightly linked within 0-3.7 cM of the Ti locus and may be useful in a marker assisted selection program.
This paper presents the design and fabrication of a micropump for cerebrospinal fluid shunt. The micropump is to be implanted in hydrocephalus patients, The micropump consist of a corrugated parylene diaphragm and a set of nozzle and diffuser. The electromagnetic force drives the diaphragm. The static or dynamic characteristics of the fabricated devices have been obtained experimentally. The size of the micropump is 14 × 12 × 8mm3. The increased flow rate of the micropump is about 50 μl/min at operational range.
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