Se ha investigado el contenido proteico y algunas actividades enzimáticas del veneno piente cascabel Crotalus durissus terrificus procedente de la región de Sandia, Puno; p empleó el veneno total así como las fracciones obtenidas mediante cromatografía de f Sephadex G-100. El porcentaje de proteína calculado por el método de Lowry fue de 68 veneno total; habiéndose obtenido 3 picos de proteína durante el fraccionamiento; en el registró actividad proteolítica, en el segundo, las actividades amidolítica, coagulante y fos mientras que en el tercero se detectó otra fracción proteolítica . No se registró la acetilcolinesterasa mientras que la actividad L-aminoácido oxidasa sólo se encontró en total.Palabras clave: Veneno , serpiente cascabel, enzimas, Puno. ABSTRACTThe venom of the rattlesnake Crotalus durissus terrificus from the region of Sandia, Puno investigated for its protein content and some enzymatic activities, using for it the whole ven as the fractions obtained by gel filtration chromatography in Sephadex G-1 OO. The protein calculated by the method of Lowry was of 68,6% for the whole venom; 3 peaks were obta the fractionation; the first showed proteolytic activity, the second, amidolytic, clotting and pase A 2 activities, while the third, another proteolytic activity. Acetylcholinesterase activity wa while L~amino acid oxidase activity was found only in the whole venom.
Micrurus spixii venom was studied after fractionation by Sephadex G-100 SF gel filtration chromatography. Several enzymatic activities and biological effects were investigated in whole venom and fractions. The venom was resolved in four peaks in a range of about 73.2-10.7 kDa molecular weight. Alkaline phosphatase and acetylcholinesterase activities were found in peak I, and procoagulant activity was seen in peak II. Phospholipase A2, hemorrhagic, and proteolytic activities were detected in peak III. A second procoagulant factor and proteinase were present in peak IV. Thrombin-like enzyme and direct hemolytic activities were not found in any assayed samples
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