Asparagine‐glycine‐arginine (NGR)‐containing peptides targeting aminopeptidase N (APN)/CD13 can be an excellent candidate for targeting ligands in molecular tumor imaging. In this study, we developed two NGR‐containing hexapeptides, and evaluated the diagnostic performance of Tc‐99 m labeled hexapeptides as molecular imaging agents in an HT‐1080 fibrosarcoma‐bearing murine model. Peptides were synthesized using Fmoc solid‐phase peptide synthesis. Radiochemical purity of Tc‐99 m was evaluated using instant thin‐layer chromatography. The uptake of two NGR‐containing hexapeptides within HT‐1080 cells was evaluated in vitro. In HT‐1080 fibrosarcoma tumor‐bearing mice, gamma images were acquired. A biodistribution study was performed to calculate percentage of the injected dose per gram of tissue (%ID/g). Two hexapeptides, glutamic acid‐cysteine‐glycine (ECG)‐NGR and NGR‐ECG were successfully synthesized. After radiolabeling procedures with Tc‐99 m, the complexes Tc‐99 m hexapeptides were prepared in high yield. The uptake of Tc‐99 m ECG‐NGR within the tumor cells had been assured by in vitro studies. The gamma camera imaging in the murine model showed that Tc‐99 m ECG‐NGR was accumulated substantially in the subcutaneously engrafted tumor. However, Tc‐99 m NGR‐ECG was accumulated minimally in the tumor. Two NGR‐containing hexapeptides, ECG‐NGR and NGR‐ECG were developed as molecular imaging agents to target APN/CD13 in HT‐1080 fibrosarcoma. Tc‐99 m ECG‐NGR showed a significant uptake in the tumor, and it is a good candidate for tumor imaging.

Two ELR-containing 6-mer peptides, ELR-ECG and ECG-ELR, were developed as a molecular imaging agent to target CXCR2 of epidermoid carcinoma. Tc-99m ELR-ECG had showed significant uptake in tumor and it was good candidate for a tumor imaging.

We successfully developed Tc-99m ECG-RRL as a new tumor imaging candidate. Specific tumoral uptake of Tc-99m ECG-RRL was evaluated both in vitro and in vivo, and it was determined to be a good tumor imaging candidate. Additionally, Tc-99m ECG-RRL effectively distinguished between cancerous tissue and inflammatory lesions.

This study demonstrated that there are substantial discrepancies between individual LBM CTs and LBM PEs, and this should be taken into account when LBM CTs and LBM PEs are used interchangeably between patients.

ObjectiveThe purpose of this study is to investigate predictors of crossed cerebellar diaschisis (CCD), and the effects of CCD on functional outcomes including motor function, activities of daily living, cognitive function, and ambulation 6 months after onset in patients with intracerebral hemorrhage (ICH).MethodsA total of 74 patients experiencing their first ICH were recruited. If the asymmetric index was more than 10% using single photon emission computed tomography (SPECT), a diagnosis of CCD was confirmed. Clinical factors were retrospectively assessed by reviewing medical records. Radiologic factors encompassed the concomitance of intraventricular hemorrhage, side and location of the lesion, and hemorrhage volume. Functional outcomes were evaluated using the Fugl-Meyer Assessment, the Korean version of the Mini-Mental State Examination, the Korean version of the Modified Barthel Index, and measurement of the Functional Ambulatory Category at the time of SPECT measurement and 6 months post-ICH.ResultsLesion location, especially in the basal ganglia (odds ratio [OR]=6.138, p=0.011), and hemorrhagic volume (OR=1.055, p=0.046) were independent predictors for CCD according to multivariate logistic regression analysis. In addition, the presence of CCD was significantly related to the improvement in Fugl-Meyer Assessment score after 6 months (adjusted R2=0.152, p=0.036).ConclusionLesion location and hemorrhagic volume were the predisposing factors for CCD, and the CCD was associated with poor motor recovery over 6 months in patients with hemorrhagic stroke.