Nowadays, the majority of microbial EPS are not produced on an industrial scale because of high cost and low yield of the target product. In this study, the cultivation conditions of Acinetobacter sp. IMV B-7005 for the efficient process of exopolysaccharide ethapolan synthesis on refined or cheaper waste oil and oil-containing mixed substrates were established. The production efficiency on the selected substrates was evaluated by the amount of synthesized ethapolan (g/l) and EPS-synthesizing ability (g EPS/g biomass). Regardless of the quality (sunflower, corn, olive, rapeseed) and type (after frying meat or potatoes) of waste oil in the biosynthesis medium, it was found that the polysaccharide synthesis and its rheological properties were at the level obtained using the refined substrate. Use of waste oil, especially mixed one, in mixture with molasses or acetate allows increasing the amount of synthesized EPS to 14-16 g/l. The obtained results show the possibility of developing a universal technology of ethapolan production on the oil-containing substrates and their mixtures, which is independent of the type and quality of the waste oil, as well as its supplier.
Introduction. The purpose of research-to compare the cost of nutrient mediums for obtaining culture fluids of Acinetobacter sp. IMB B-7005 and Xanthomonas campestris strains (xanthan gum producers) for its using in the secondary oil extraction. Materials and methods. Calculation of the culture fluid amount and the cost of nutrients for the ethapolan (producer-Acinetobacter sp. IMB B-7005) and xanthan (producers of Xanthomonas campestris strains) exopolysaccharides production as oil extraction agents for 262 oil wells of Oil and Gas Production Administration "Okhtyrkanaftogas" of JSC "Ukrnafta" was carried out taking into account the following parameters:4 times treatment per year of wells with the 15 m 3 of EPS solution with its concentration 0,05%). Results and discussions. On the basis of the data on the concentration of synthesized polysaccharides by strainsproducer, the annual need for a culture fluid (545-1849 m 3) was calculated for the treatment of wells of the OGPA "Okhtyrkanaftogas" and the amount of product per fermentation cycle according to selected technologies. Theoretical calculations have shown that costs for the nutrient medium preparing for Acinetobacter sp. IMB B-7005 culture fluid obtaining on waste sunflower oil after frying meat, necessary for increasing secondary oil extraction at "Okhtyrkanaftogas" wells in 1.8-5.4 times less than in the case of ethapolan obtaining on C2-C6 substrates, molasses or their mixtures. Comparison of nutrient medium cost for the preparation of ethapolan and the most famous polysaccharide xanthan obtained on molasses on technical glycerine was made. The cost of polysaccharide xanthan obtaining (in amount that would provide oil extraction in equivalent quantity like in case of ethapolan using) in 12.7-16.7 times higher compared to the ethapolan obtaining. Conclusion. Obtained results confirm the economic feasibility of the ethapolan synthesis on waste oil for the polysaccharide using in secondary oil production in comparison with the technologies for xanthan obtaining.
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