Immobilization of enzymes is a good field of study to expand the life of enzyme in and lowering the cost of the chemical processes such as separation processes. Urease is an important enzyme with medical and industrial applications. The aim of the present study is to prepare an immobilized urease on a strong cation exchange resin (Amberlite IR120 -Na) and study of its activity and stability. We monitored the liberation of Na ions in the collected fractions and searching for protein in the fractions as an indicators of immobilization by ion exchange phenomenon. Sodium is measured using atomic absorption spectroscopy techniques, while protein tested by Bradford’s method. Immobilized urease activity was evaluated by salicylate-hypochlorite method. The results indicated a complete immobilization of urease enzyme on the resin surface with reserving 92% of the activity of free enzyme. The immobilized urease enzyme on resin showed good stability and it has a 62% of its activity after 154 days of storage at room temperature. It is concluded that a new immobilized urease enzyme system is prepared with good enzyme activity and stability.
In this work the use of a mobile phone as a spectrophotometer using camera resolution by installing the software (application store AAP) on the phone (i Phone 6), which analyzes the colour images (RGB) in results with a colour length where it was possible to calculate the colour value of each image representing a specific concentration of the solution under study. A calibration curve with a range of (1 × 10-3 - 6.25 × 10-4) mmole.L-1 using optical image analysis with the concentration of the preparation of potassium permanganate (KMnO4). A calibration curve for statistical correlation range of 0.993 (R2) was found.
Different products from a unique Propolis extract, Propolis (bee glue), of resinous consistency produced by bees, has been used as indigenous medicine for the treatment of several diseases in some contrary. Safety assessment of propils extract with respect to antimicrobial activity against three bacterial isolates, Proteus Mirabilis, Klebsiella Pneumonia and Staphylococcus Qureus. The result showed that the main composition of the extract was Anthraquinone, the last one was responsible for biological activity of the extract. FT-IR, UV results showed more than eight peaks for represent the main fine composition of the extract. The present study also showed that the extract has antibacterial activity against three bacterial isolates
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