Growth and development of the pinna in three species N. cordifolia, N. exalta(i), N. exalta(ii) and N. furcans were viewed in the department of botany of Obafemi Awolowo University, IIe-Ife campus. The results showed that all the venation of the pinnae of the species consist of a mid-vein and 2 lateral series of dichotomous vein all of which terminate freely near the lamina margins. Because it seems their venation patterns are similar, this might be one of the reasons for grouping them into one genus called Nephrolepis Schott. The development and growth pattern of N. cordifolia and N. furcans was uniform and consistent, while that of N. exalta gave two forms as the young was represented N. exalta (i) and the matured as N. exalta (ii). The growth patterns showing variations in the mid-rib, fertile and sterile pinnae provided useful specific distinctions.
The present study aimed to evaluate antimicrobial activity of ethanol, methanol, schnapp (40% alcohol), oil palm wine and Raffia palm wine extracts of moss species Archidium ohioense, Pelekium gratum and Hyophila involuta against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Candida pseudotropicalis. The antimicrobial activities of the alcoholic extracts were tested against selected microorganisms using agar well diffusion method. Minimum inhibitory concentrations (MIC) of the extracts were determined using standard methods. The antimicrobial test of the extracts on selected organisms revealed that the methanolic and ethanolic extracts of the mosses studied were inactive against all the bacteria and fungi screened, while the schnapp, Oil palm wine and Raffia palm wine extracts showed significant activity against the selected organisms. The minimum inhibitory concentration (MIC) value of the extracts on the test organisms ranged from 1.25 to 40 mg/ml. The study concluded that the extracts of the mosses studied contain pharmacologically active constituents which may be responsible for their antimicrobial properties.
The present study compared the extraction of chlorophylls from selected forest mosses (Hyophila involuta and Thuidium gratum) and derived savanna moss (Archidium ohioense) using dimethylsulphoxide (DMSO) and 80% acetone. The mosses were collected from natural populations found in the Central Campus of the Obafemi Awolowo University, Ile-Ife, Nigeria. The chlorophyll extractions process followed standard methods and the absorbance of the extracts were read on spectrophotometer at wavelengths of 645 nm and 663 nm respectively. The data obtained were later subjected to appropriate statistical analysis. The results showed that DMSO was a better chlorophyll extractant for mosses than 80% acetone. Although there were significant differences in the chlorophyll a, chlorophyll a/b ratio and total chlorophyll accumulation within all three species using DMSO and 80% acetone as extractant (P < 0.05), there was no significant difference in the chlorophyll b accumulation of all the three species (P > 0.05).
Analyses of the constituents of the crude extracts obtained from young and mature moss species namely: Archidium ohioense and Philonotis hastata were conducted with a view to investigating the effects of maturity stages on their bioactive constituents. The mosses collected from their natural population were air dried at ambient temperature in the laboratory, extracted with methanol and the crude extracts subjected to gas chromatographymass spectrometry (GC-MS) analysis. The results of the analyses showed the presence of 20 compounds in young A. ohioense with n-hexadecanoic acid [26.60%], bis(2-ethylhexyl) phthalate [12.47%], bicyclo (3.1.1)heptane 2,6,6-trimethyl-[1r-(1.alpha.,2.beta.,5.alpha.)]- [11.59%] and phytol [9.69%] forming the prominent components while in the mature A. ohioense, 13 compounds were present, from which n-hexadecanoic acid [51.25%], hexadecanoic acid 2-hydroxy-1-(hydroxymethyl)-ethyl ester [9.90%] and n-propyl 9-octadecenoate [7.47%] formed the prominent components. In P. hastata, 20 compounds were identified in the young stage sample with n-hexadecanoic acid [22.46%], bis(2-ethylhexyl) phthalate [20.95%] and phytol [18.14%] as the prominent components while 9 compounds were identified in the mature sample with n-hexadecanoic acid[51.84%], hexadecanoic acid 2-hydroxy-1-(hydroxymethyl)ethyl ester [18.12%] and bis(2-ethylhexyl) phthalate [9.11%] which formed the prominent components. The study indicated that, maturity stages at collection of the mosses affected their bioactive compositions, with the young stage mosses showing more bioactive compounds than the mature ones. Keywords: A. ohioense, P. hastata, Mosses, Maturity stages, Crude extract, GC-MS.
The phytochemical screening of Hyophila involuta collected from the Biological Garden of the Obafemi Awolowo University, Ile-Ife, Nigeria, was carried out to investigate the presence or absence of some secondary metabolites and its antibiotic potentials, using different extracts (with acetone and ethanol) on selected organisms. The extracts obtained were screened for the presence of secondary metabolites like alkaloids, anthraquinones, cardiac glycosides, flavonoids, phlobatanins, saponins, steroids, tannins, triterpenes and xanthoproteins. Antimicrobial activity of the extracts was carried out on Escherichia coli, Staphylococcus aureus, Aspergillus flavus and Candida albicans. Only acetone extract tested positive for the presence of flavonoids, while alkaloids and cardiac glycosides were detected present in both the acetone and ethanolic extracts. Flavonoids were detected present only in the acetone extract. Saponins were detected present only in the ethanolic extract. The extracts (acetone and ethanolic) showed significant activity on Staphylococcus aureus and Aspergillus flavus. The results obtained from this study indicated that H. involuta has medicinally important compounds, having therapeutic potential from which effective antimicrobial medicine can be sourced.
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