Anatolian populations inhabit the southern latitudinal margin of species distribution ranges and therefore may be considered as “rear edge” populations. The genetic structures of such populations have critical importance in species responses to climatic change and are essential for long-term conservation genetics. Here, the genetic structure of Chorthippus parallelus (Zetterstedt) (Orthoptera: Acrididae: Gomphocerinae) populations in one of the main southern glacial refugium is investigated. Ten populations of C. parallelus from Anatolia have been studied by investigating single-copy nuclear DNA (Cpnl-1) fragment using polymerase chain reaction-single strand conformational polymorphism methods. The level of allelic number was high (total of 20 alleles in the locus, of which 11 were unique), but the level of gene flow among populations seemed to be low (FIS = 0.090). However, most populations were genetically diverse (HE > 0.5, A = 6.211, and ne = 5.774). The level of genetic differentiation among populations was high (FST = 0.330). No statistically significant correlation between genetic diversity and spatial distribution was observed. The analysis of molecular variation analysis indicated that a large proportion of genetic variation was due to differentiation among individuals within populations. Genetic drift was a more likely cause of differentiation among populations rather than geographical distance. These results suggest that a presence of a “stable rear edge” population in contrary to the center-periphery model.
Further elucidation of the molecular mechanisms underlying lung cancer (LC) is essential for the development of new effective therapeutic agents. Recently, involvement of Wnt antagonists in oncogenesis has been demonstrated in several cancers. The investigation of their contribution to lung carcinogenesis is still under investigation. We aimed to investigate whether there is a susceptibility or preventive effect of Wnt antagonist gene polymorphisms on the development and/or prognosis of LC. We investigated 110 LC patients and 160 controls. Single-nucleotide polymorphisms of Wnt antagonist genes including DKK2 (rs17037102), DKK3 (rs3206824), DKK3 intron4 G/C (rs7396187), DKK4 (rs2073664), and sFRP4 (rs1802074) were analyzed using nested polymerase chain reaction and restriction fragment length polymorphism. Results showed that patients with DKK3 AA compared with controls have a decreased risk of LC (adjusted for smoking habit, body mass index, and familial history) (P = 0.02; odds ratio [OR],0.08; 95% confidence interval [95% CI], 0.01-0.7). It was found that, for sFRP4 polymorphism, patients with GG and GA genotypes versus AA genotype controls showed a decreased risk for LC (P = 0.01; [OR, 0.19; 95% CI, 0.05-0.73 for GG genotype]; [OR = 0.18, 95% CI, 0.04-0.72 for GA genotype]). In addition, a decreased risk of LC was also found for the genotype combination of DKK3 (rs3206824) GG and sFRP4 AG + GG (P = 0.004; OR, 0.12; 95% CI, 0.02-0.58). We suggest that these 2 polymorphisms have a protective effect on LC in this study.
Familial Mediterranean fever (FMF) is the most frequent hereditary inflammatory disease characterized by self-limited recurrent attacks of fever and serositis. The aim of the current study is to determine the frequency of the mutations in 365 suspected FMF patients and to reveal whether there is a correlation between genotype and phenotype of these patients. All patients were clinically examined according to Tell-Hashomer FMF criteria and were screened genetically in terms of common 12 Mediterranean fever gene (MEFV) mutations. Various point mutations were detected in 270 (74%) patients. The most frequent mutation was M694V (26.85% of the alleles) and was followed by E148Q (15.55%), M680I (G/C) (9.62%) and V726A (7.96%). Patients who bear M694V homozygous mutation had most severe disease phenotype and high risk for amyloidosis (P = 0.04). Our results indicate that Sivas population has a wide range of heterozygous mutated carriers of MEFV gene and there is a high frequency of E148Q allele when compared to the other Mediterranean groups.
Introduction:Association betweenMethods:Total 200 subjects including 100 patients and 100 controls were analyzed and used polymerase chain reaction and restriction fragment length polymorphism methods for genotyping analysis of the polymorphism.Results:We found that smokers compared with non-smokers have approximately eight fold higher lung cancer risk [p=0.0001, OR=8.27 (4.02–16.9)]. Frequency of GG genotype was higher in patients than in controls, but this ratio was not significant (χConclusion:No correlation between
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