Background: Soleshine is a polyherbal formulation meant to be used to heal cracks on heels, protecting sole from chaffs, marketed by Rumi Herbal Pvt Ltd., Chennai. The active ingriedients are Azadirachta indica, Lawsonia alba, Shorea robusta, Ricinus communis, and Sesamum indicum, which were well known for their antimicrobial activity. Aim and Objectives: The aim of this study is to evaluate the antifungal activity of the ingredients and to evaluate the synergistic activity present in the preparation. Materials and Methods: The plant materials were procured, dried, powdered, and sequentially extracted. The organisms were collected and evaluated the antifungal activity with disc diffusion method for all individual extracts and in combination of different extracts. Sabouraud dextrose agar, culture medium was used for the growth of fungal organism. The minimum inhibitory activity was observed with resazurin method. Clotrimazole, an antifungal drug, belongs to azole group was taken as a standard negative control. Results: The zone of inhibition was determined by disc diffusion method where it shows the inhibition of growth of fungal organism. The same experiment was repeated totally for 6 times and analyzed with SPSS software in May 2017, found that significant (P < 0.05). The minimum amount of extract required to inhibit the growth was determined by resazurin dye method where the color change (oxidation-reduction reaction) implies the viability of fungi. Conclusion: The formulation has an antifungal activity in all the extracts and maximum activity was with aqueous extract, and also, it shows synergistic activity in combination which quotes the additive effect.
A stability indicating reverse phase ultra performance liquid chromatography (UPLC) for perampanel and identification of degradation products of perampanel from forced degradation studies. Perampanel was subjected to stress conditions of acid, aqueous hydrolysis, oxidative, photolytic and thermal stress degradation. The degradation of perampanel was observed under acid hydrolysis, basic hydrolysis and peroxide and it was stable in thermal, photo degradation conditions. Good resolution was observed between drug and its degradation products with the proposed UPLC method was developed by using mobile phase formic acid in water and acetonitrile. The developed UPLC method was validated with respect to specificity, linearity, accuracy, precision, ruggedness and robustness. The linearity of method was performed concentration levels between 20 to 120 µg/mL with correlation coefficient 0.999. Assay recoveries were found to be 98.2 to 101.6 %. The developed UPLC method was found to be useful to determine the regular production of perampanel samples and its stability studies. Two new degradants were formed (1-2). Both acid and base hydrolysis resulted in the same compound 1. However, oxidation by peroxide resulted in another new compound 2. Both degradation products were completely characterized by HRMS and extensive NMR, spectrometry. Stability indicating validated method by UPLC and its degradation and cartelization studies were not reported elsewhere.
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