Plant tissue culture techniques are useful for producing a large number of disease-free plant material. The aim of the present study was to determine the optimal nodal explant type (terminal, intermediate and basal) and cytokinins (6-benzyladenine and thidiazuron [TDZ] at concentrations of 0 to 3 mg L -1 ) for bud break and axillary shoot proliferation of Glycyrrhiza glabra L. The intermediate nodal explants were effective for the establishment of G. glabra aseptic culture when compared with terminal or basal nodal explants. The highest bud break (89%) and axillary shoot proliferation (eight shoots) were observed when the intermediate nodal explants were cultured on MS medium supplemented with 2 mg L -1 TDZ for 30 d. The best rooting medium was MS medium supplemented with naphthalene acetic acid (NAA) compared with indole acetic acid; NAA at 6 mg L -1 yielded 78% rooting and an average of 6.3 roots per plantlet. The regenerated plantlets were acclimatized, with a survival rate of 77.7%, when transferred to ex vitro conditions and showed no morphological abnormalities. Thus, the high percentage of bud break and rate of shoot proliferation and successful acclimatization established a simple and efficient micropropagation of G. glabra.

Diabetes is a condition of the body in which blood sugar level is higher than the normal average value. It is considered as a major

Kandungan hara yang tinggi pada sludge industri penyamakan kulit menjadikan dasar untuk mengeksploitasi sludge tersebut sebagai campuran media tanam pada pucuk merah. Penelitian bertujuan untuk mengetahui kemampuan tanaman pucuk merah terhadap pemberian sludge IPAL industri penyamakan kulit dan mengetahui konsentrasi yang dapat digunakan sebagai campuran media tanam pada tanaman pucuk merah. Variasi komposisi sludge:tanah, yaitu 5%:95%, 10%:90%, 15%:85%, 20%:80%, 0%:100%. Penelitian ini dilakukan dalam waktu 3 minggu dengan frekuensi pengamatan 3 hari sekali terhadap tinggi dan diameter batang. Hasil penelitian menunjukkan bahwa Syzygium oleana dapat bertahan hidup dari awal sampai akhir penelitian dengan pemberian konsentrasi sludge yang sesuai karena kandungan unsur hara yang tinggi sehingga mempengaruhi kesuburan tanaman baik dalam pertumbuhan tinggi dan perkembangan diameter tanaman yang meningkat dibandingkan dengan tanaman kontrol. Konsentrasi sludge yang dapat digunakan sebagai campuran media tanam pada tanaman pucuk merah berada pada konsentrasi di bawah 50%. Kata kunci: Industri Penyamakan Kulit, Sludge IPAL, Syzygium oleana

The objective was to review and add literature whether green tea is helpful for weight reduction. Reviewing the randomized, double-blind, placebo-controlled