The study was conducted in the laboratories of the College of Education for Pure Sciences for the academic year 2021-2022 on fungi that produce aflatoxin toxin that were isolated from some foodstuffs traded in the local markets in the city of Karbala and were diagnosed molecularly using a technique Polymerase chain reaction (PCR) which successfully doubled NS8 and NS1 with prefixes (SSU-) The same primers were used to determine the sequence of nitrogenous bases of rRNA belonging to the International Center for Information Technology Genbank. National Center of Biotechnology Information (NCBI) it has been deposited in a database (fungi biogenic). The results of isolation, which were collected from imported and local foodstuffs, were contaminated with fungi, and that all isolated fungi were producing aflatoxin toxin. This was inferred by using coconut medium with ammonia solution. hf ifheehehai eifenhihehidnhf eht dna Penicillium oxalicum, Penicillium expansum, Aspergillus flavus, Cladosporium Uredinicola, Aspergillus sydowii, Aspergillus Oryzae, Aspergillus Tamarii, Aspergillus nomius, Alternaria triticina, Penicillium griseofulvum, Aspergillus austwicki, Aspergillus flavus, Cladosporium cladosporioides, Actinomucor elegans, Penicillium brevicompatum, Trichophyton mentagrophytes, Gibberella intermedia, Debaryomyces hansenii, Aspergillus caespitosus, Aspergillus versicolor.
This study was conducted in the Department of Life Sciences / College of Education for Pure Sciences / University of Karbala, where the effects of the alcoholic and aqueous extract of cloves on 21 aflatoxin-producing fungi isolated from foodstuffs were studied. 2.5 If compared to the comparison treatment and treatments in which concentrations of water and alcoholic extracts of cloves were used, if the alcoholic extract of cloves showed a high effectiveness in inhibiting the tested fungi, it was inhibitory for all fungi producing aflatoxin at a concentration of 2.5 mg/ml. As for the aqueous extract only, the results of the statistical analysis at the probability level of 2.5 mg / ml, there were clear significant differences between the tested fungi and high significant differences between The comparison treatment and the concentrations used for the extract Watery carnation The results of the study also showed that the alcoholic extract is more effective in inhibiting the growth of fungi than the aqueous extract.
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