The sanitation control of pathogens in the tropical effluents needs much more attention to ensure ecosystem health integrity and the safety of human health. The common use of chemicals in achieving this in wastewater treatment has remained unsustainable due to much health concern. Indeed, based on the numerous challenges associated with faecal pathogenic bacteria in wastewaters, the focus is now on achieving higher purification efficiencies in the elimination of the human pathogens from wastewater through eco-sustainable systems such as constructed wetlands (CWs). Hence, the need to explore the application of constructed wetlands in wastewater treatment under specific local environmental conditions for accurate understanding and improved treatment efficiency. This study therefore aimed at monitoring constructed wetlands faecal bacteria purification efficiency through integrated non-molecular membrane filtration technique and molecular quantitative polymerase chain reaction (MFT-qPCR) technique. The results showed some shortfall in the treatment system and also proved that integrating MFT-qPCR in faecal bacterial purification monitoring within a constructed wetland system provides a more accurate and reliable outcome. Additionally, the wetland purification efficiency was low (<80%) with the dissolved oxygen posing the strongest influence on faecal pathogenic bacterial purification trend across the wetland. Hence, the need to regularly carry out dredging and macrophyte harvesting as well as the use of holistic and more integrative approaches such as MFT-qPCR in managing and monitoring the performance of CWs in faecal pathogen eradication for improved CWs purification efficiency.
As in other parts of Africa, and in other developing nations, the rise in the human population and anthropogenic activities within the Lake Naivasha basin is causing an increase in human health risks due to faecal contamination of domestic water sources. This study investigated faecal pollution of community water sources within the Lake Naivasha basin by measuring the densities of total coliforms, Escherichia coli, intestinal enterococci, Clostridium perfringens and heterotrophic bacteria in Lake Naivasha, the Malewa and Gilgil Rivers, and boreholes using membrane filtration techniques and heterotrophic plate count procedures. Selected physico-chemical parameters were also measured in situ from all the water sources sampled. Lakes and rivers had significantly higher microbial abundances than boreholes. Unlike boreholes, surface sources (rivers and lake) showed significant variation with respect to sampling sites for all the microbiological parameters (P < 0.05). The use of solar radiation in water disinfection with temperatures of 75 °C after 30 minutes from pasteurization point (time zero) fully eradicated E. coli and total coliforms from all the water sources. In conclusion, there is faecal pollution in water sources used by communities within the Lake Naivasha basin. The use of solar radiation is therefore recommended for water purification to reduce likely incidences of waterborne diseases.
Egerton University (EU) uses Wastewater Stabilization Ponds (WSPs) for wastewater treatment. An adjoining wetland with gravel bed substrate and freesurface flow meant to polish discharge before releasing into River Njoro is currently non-operational. The current study aimed at establishing the performance of WSPs in terms of concentration and reduction efficiencies of Faecal Indicator Bacteria (FIB); Total Coliforms (TC) and Escherichia coli (E. coli). Wastewater samples were collected weekly for one month from mid-November to mid-December 2017. Total Coliforms and E. coli were isolated using selective and differential media following Membrane Filtration (MF) method. Colonies were cultured on Chromocult Coliform Agar (CCA) and enumerated using standard procedures for examination of water and wastewater. The results were expressed as Colony Forming Units (CFUs) per 100 ml of the original sample. The inlet showed highest concentration of FIB which reduced along the treatment pathway. Total Coliforms and E. coli ranged between 2.5 × 10 8 -2.9 × 10 11 and 5.9 × 10 5 -1.8 × 10 10 CFUs/100 ml respectively with cumulative reduction efficiencies between 2 to 4 log units for E. coli and 2 to 3 log units for TC in the two maturation ponds. Although concentration of FIB in EU WSPs reduces along the treatment pathway, the effluent quality is above recommended international standards for discharge into surface waters. The trend is attributed to lack of frequent monitoring, insufficient maintenance, together with short-circuiting effect due to by-passing of wastewater in the design of the new pond system.
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