--Introduction. Two Turkish fig cultivars, Alkuden and Bursa Siyahi, were propagated by meristem culture to eliminate the fig mosaic virus. The technique of dsRNA analysis was conducted on the in vitro propagated plants to test for virus-free status. Materials and methods. Four different Murashige and Skoog (MS) media complemented with different concentrations of growth hormones (GA 3 , BA and IBA) were compared to study the cultured meristems' survival rate, shoot formation and rooting. Short-and long-term thermotherapy treatments were also applied. Results. For survival rate, 0.2 mg GA 3 ·L -1 + 0.5 mg BA·L -1 gave the most favorable results; for shoot formation, it was the medium with 0.2 mg GA 3 ·L -1 + 2.0 mg BA·L -1 which was the best, while rooting rate was the highest for meristems cultured on MS medium with only 0.1 mg GA 3 ·L -1 . Conclusion. Meristem culture, together with thermotherapy treatment, is recommended to obtain virus-free fig plant material. Although cultured plants seemed to be very healthy, dsRNA virus tests are recommended for sensitive evaluation of the sanitary status of the plants obtained.
Detection of Tospoviruses in Ornamental Plants in Hobby Gardens and Landscaping Areas in Adalar District of Istanbul Province
A R T I C L E I N F O A B S T R A C T
Research ArticleReceived 30 During the surveys carried out in that district, the samples were collected from both simptomatologically suspicious ornamental plants and the plants which did not show any symptoms. All of the collected samples (n=150) were firstly tested by Double Antibody Sandwich (DAS) ELISA and none of the samples were found to be infected with TSWV and INSV. The samples detected to be positive with IYSV by ELISA tests were then used in RT-PCR studies. At the RT-PCR using the IYSV-465c; IYSV-239f primer pair, a band with a size of 240 bp was observed for Pittosporum tobira and Hydrangea macrophylla. Therefore, the presence of IYSV infection in Adalar was also confirmed molecularly.
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