No abstract

The aim of this study was to investigate the effects of red dragon fruit (Hylocereus polyrhizus) extract (DFE) on the stomach in ulcer model induced by indomethacin in rats. Effects of DFE were evaluated in indomethacin-induced gastric damage model on Sprague-Dawley rats. Experimental model: all rats were fasted for 24 h. At the end of this period, DFE was administered to the ulcer-induced groups. One hour after this application, a dose of 25 mg/kg of indomethacin was applied by oral gavage to all groups except the HEALTHY and DFE1000 groups. Six hours after indomethacin administration, the rats were euthanized with high-dose anesthesia and the experiment was terminated. Macroscopic and microscopic analyses for investigating ulcerative area, molecular and biochemical analyses for oxidative damages investigation and molecular analyses for the effect mechanism of indomethacin and DFE were conducted on stomach tissues in the study. While oxidative stress-associated markers such as MDA, BAX, and Caspase 3 increased dramatically in the indomethacin group, GSH antioxidant levels decreased. It was observed that these parameters were significantly improved in DFE 500 mg/kg and DFE 1000 mg/kg groups compared to ulcer group, and the results of especially DFE 1000 mg/kg group were similar to famotidine group.We observed that our histopathological findings also supported all our other findings.Dragon fruit extract was protected against indomethacin-induced ulcer damage by decreased MDA levels, increased GSH levels, and inhibition of Caspase 3, BAX, and Cox-2, and activation of Cox-1.

ÖzPurpose: In this study, our aim was to investigate the potential effects of strong antioxidant daidzein (DZ) on ovarian ischemia and reperfusion injury. Materials and Methods: A total of 42 female Sprague-Dawley rats were randomly divided into seven groups. For the experimental model, the clamps were removed after 3 hours of ischemia, and blood flow was provided again. Then, reperfusion process was terminated for 3 hours. Daidzein was orally administered to animals at doses of 35 and 70 mg/kg 30 minutes before ischemia (I) and ischemia and reperfusion (I/R) procedures. Results: Severe immunoreactivity of the IL-1β, IL-6 and Caspase-3 were detected in I and I/R groups. Moderate immunoreactivity of IL-1β, IL-6 and Caspase-3 was detected in I+DZ35 and I/R+DZ35 groups, and slightly positivity was detected in I+DZ70 and I/R+DZ70 groups. The SOD activity level increased in the groups treated with Daidzein, while MDA levels decreased. In addition, hemorrhage areas and inflammatory cell migration decreased in I/R+DZ70 and I/R+DZ35 groups, when compared to I/R group in a dose dependent manner. Conclusion: Daidzein has a strong protective role in the treatment of ovarian ischemia-reperfusion injury and can be used as a therapeutic agent.Amaç: Bu çalışmada, güçlü antioksidan daidzeinin (DZ) over iskemi ve reperfüzyon hasarı üzerindeki potansiyel etkisini araştırdık. Gereç ve Yöntem: Toplam 42 adet dişi Sprague-Dawley sıçan rastgele yedi gruba ayrıldı. Deney modeli için, 3saatlik iskeminin ardından klempler çıkarıldı ve tekrar kan akışı sağlandı. Reperfüzyon 3 saatin sonunda sonlandırıldı. Daidzein, iskemi (I) ve iskemi ve reperfüzyon (I/R) işlemlerinden 30 dakika önce hayvanlara ağızdan 35 ve 70 mg/kg dozlarında uygulandı. Bulgular: I ve I/R gruplarında IL-1β, IL 6 ve Caspase 3'ün şiddetli immünoreaktivite görüldü. I+DZ35 ve I/R+DZ35 gruplarında IL-1β, IL 6 ve Kaspaz 3'ün immünreaktivitesi orta düzeyde iken, I+DZ70 ve I/R+DZ70 gruplarında hafif pozitiftir. Daidzein ile tedavi edilen gruplarda SOD aktivite seviyesi artarken, MDA seviyeleri azaldı. Ayrıca I/R + DZ70 ve IR+DZ35 gruplarında I/R grubuna göre doza bağlı olarak kanama alanları ve inflamatuar hücre göçü azaldı. Sonuç: Daidzein, over iskemi-reperfüzyon hasarının tedavisinde güçlü bir koruyucu role sahiptir ve terapötik bir ajan olarak kullanılabilir.

Paracetamol is one of the drugs that cause hepatic damage. Fisetin has wide pharmacological effects such as anticancer, antiinflammatory and antioxidant. We aimed to evaluate the possible protective effect of fisetin on paracetamol-induced hepatotoxicity. Fisetin was administered at 25 and 50 mg/kg doses. Paracetamol was administered orally at a dose of 2 g/kg for induce hepatotoxicity 1 h after the fisetin and NAC treatments. The rats were sacrificed 24h after the Paracetamol administration.Tumor necrosis factor-alpha (TNF-α), NFκB and CYP2E1 mRNA levels and Superoxide dismutase (SOD) activity, glutathione (GSH) and malondialdehyde (MDA) levels of livers were determined. Serum ALT, AST and ALP levels were measured. Histopathological examinations were also performed. Fisetin administration significantly decreased the ALT, AST and ALP levels in a dose dependent manner. In addition, SOD activity and GSH levels increased, and the MDA level decreased with the treatment of fisetin. The TNF-α, NFκB and CYP2E1 gene expressions were significantly lower in both doses of the fisetin groups compared with the PARA group. Histopathological examinations showed that fisetin has hepatoprotective effects. This study showed that fisetin has the liver protective effects by increasing GSH, decreasing inflammatory mediators and CYP2E1.

No abstract

This prospective cross-sectional study, aimed to evaluate lymphocyte DNA damage in Coronavirus disease (COVID-19) patients. In this study, 50 COVID-19 positive patients attending Erzurum City Hospital Internal Medicine Outpatient Clinic and 42 control group patients were included. DNA damages were detected in living cells by lymphocyte isolation in 50 COVID-19- positive patients using the comet assay method. DNA tail/head (olive) moments were evaluated and compared. White blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), neutrophil (NEU), lymphocyte (LYM), eosinophil (EO), monocyte (MONO), basophil (BASO), platelet (PLT), neutrophil/lymphocyte ratio (NLR). The RBC, lymphocyte, eosinophil, and monocyte means were significantly higher in the control group (p < 0.05). Whereas HGB and neutrophile means were significantly higher in the study group (p < 0.05). There were significant negative correlations between COVID-19 and RBC (r = -0.863), LYM (r = -0.542), EO (r = -0.686), and MONO (r = -0.385). Meanwhile, there were significant positive correlations between COVID-19 and HGB (r = 0.863), NEU (r = 0.307), tail moment (r = 0.598), and olive moment (r = 0.582). Both the tail and olive moment mean differences were significantly higher in the study group with higher ranges (p < 0.05). COVID-19 infection statistically significant is increasing both the tail and olive damage percentage in patients, causing DNA damage. Lastly, the NLR rate was associated with the presence and progression of COVID-19.

This prospective cross-sectional study aimed to evaluate leukocyte DNA damage in coronavirus disease (COVID-19) patients. In this study, 50 COVID-19-positive patients attending the Erzurum City Hospital Internal Medicine Outpatient Clinic and 42 control group patients were included. DNA damage was detected in living cells through leukocyte isolation in 50 COVID-19-positive patients using the comet assay method. DNA tail/head (olive) moments were evaluated and compared. White blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), neutrophils (NEU), lymphocytes (LYM), eosinophils (EO), monocytes (MONO), basophils (BASO), platelets (PLT), and the neutrophil/lymphocyte ratio (NLR) were analyzed. The RBC, lymphocyte, eosinophil, and monocyte means were significantly higher in the control group (p < 0.05), whereas the HGB and neutrophile means were significantly higher in the study group (p < 0.05). There were significant negative correlations between COVID-19 and RBC (r = −0.863), LYM (r = −0.542), EO (r = −0.686), and MONO (r = −0.385). Meanwhile, there were significant positive correlations between COVID-19 and HGB (r = 0.863), NEU (r = 0.307), tail moment (r = 0.598), and olive moment (r = 0.582). Both the tail and olive moment mean differences were significantly higher in the study group, with higher ranges (p < 0.05). COVID-19 infection caused statistically significant increases in both the tail and olive damage percentage in patients, causing DNA damage. Lastly, the NLR rate was associated with the presence and progression of COVID-19.

Background: This prospective cross-sectional study, aimed to evaluate lymphocyte DNA damage in COVID-19 patients. In this prospective cross-sectional prospective cohort study, 50 COVID-19- positive patients attending Erzurum City Hospital Internal Medicine Outpatient Clinic and 42 control group patients were included. Methods: DNA damages were detected in living cells by lymphocyte isolation in 50 COVID-19- positive patients using the comet assay method. DNA tail/head (olive) moments were evaluated and compared. White blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), neutrophil (NEU), lymphocyte (LYM), eosinophil (EO), monocyte (MONO), basophil (BASO), platelet (PLT), neutrophil/lymphocyte ratio (NLR). Results: The RBC, lymphocyte, eosinophil, and monocyte means were significantly higher in the control group (p < 0.05). Whereas HGB and neutrophile means were significantly higher in the study group (p < 0.05). There were significant negative correlations between COVID-19 and RBC (r = -0.863), LYM (r = -0.542), EO (r = -0.686), and MONO (r = -0.385). Meanwhile, there were significant positive correlations between COVID-19 and HGB (r = 0.863), NEU (r = 0.307), tail moment (r = 0.598), and olive moment (r = 0.582). Conclusion: Both the tail and olive moment mean differences were significantly higher in the study group with higher ranges (p < 0.05). COVID-19 infection statistically significant is increasing both the tail and olive damage percentage in patients, causing DNA damage. Lastly, the NLR rate was associated with the presence and progression of COVID-19.