BackgroundNewcastle disease (ND) is one of the most deadly diseases of poultry around the globe. The disease is endemic in Pakistan and recurrent outbreaks are being reported regularly in wild captive, rural and commercial poultry flocks. Though, efforts have been made to characterize the causative agent in some of parts of the country, the genetic nature of strains circulating throughout Pakistan is currently lacking.Material and methodsTo ascertain the genetics of NDV, 452 blood samples were collected from 113 flocks, originating from all the provinces of Pakistan, showing high mortality (30–80%). The samples represented domesticated poultry (broiler, layer and rural) as well as wild captive birds (pigeons, turkeys, pheasants and peacock). Samples were screened with real-time PCR for both matrix and fusion genes (1792 bp), positive samples were subjected to amplification of full fusion gene and subsequent sequencing and phylogenetic analysis.ResultsThe deduced amino acid sequence of the fusion protein cleavage site indicated the presence of motif (112RK/RQRR↓F117) typical for velogenic strains of NDV. Phylogenetic analysis of hypervariable region of the fusion gene indicated that all the isolates belong to lineage 5 of NDV except isolates collected from Khyber Pakhtunkhwa (KPK) province. A higher resolution of the phylogenetic analysis of lineage 5 showed the distribution of Pakistani NDV strains to 5b. However, the isolates from KPK belonged to lineage 4c; the first report of such lineage from this province.ConclusionsTaken together, data indicated the prevalence of multiple lineages of NDV in different poultry population including wild captive birds. Such understanding is crucial to underpin the nature of circulating strains of NDV, their potential for interspecies transmission and disease diagnosis and control strategies.

This study reports the thermal analysis and species transport to manifest non-Newtonian materials flowing over linear stretch sheets. The heat transfer phenomenon is presented by the Cattaneo–Christov definition of heat flux. Mass transportation is modeled using traditional Fick’s second law. In addition, the contribution of Joule heating and radiation to thermal transmission is also considered. Thermo-diffusion and diffusion-thermo are significant contributions involved in thermal transmission and species. The physical depiction of the scenario under consideration is modeled through the boundary layer approach. Similar analysis has been made to convert the PDE model system into the respective ODE. Then, the transformed physical expressions are calculated for momentum, thermal, and species transport within the boundary layer. The reported study is a novel contribution due to the combined comportment of thermal relaxation time, radiation, Joule heating, and thermo-diffusion, which are not yet explored. Several engineering systems are based on their applications and utilization.

The mathematical modeling of hybrid nanofluid flow and heat transfer with entropy generation toward parabolic trough surface collector (PTSC) inside the solar-powered ship (SPS) is performed. The mathematical model used non-Newtonian Oldroyd-B model amidst a constant inclined magnetic field influence is being considered. The mathematical model is then reduced by adopting appropriate similarity transformation into a higher-order nonlinear differential equations system. The reduced model is computed using the well-known technique called the Keller Box scheme. Physical parameters effectiveness, for instance, thermal radiation, viscous dissipation, hybrid nanoparticles, and Joule heating, is displayed in graphs. The silver-ethylene glycol (Ag-EG) characteristic performance outperformed the silver-magnetite-ethylene glycol (Ag-Fe3O4/EG). The maximum efficiency of Ag-EG is about 26.3%, while the minimum is at least 5.6%.

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In this article, thin film flow of non-Newtonian pseudo-plastic fluid is investigated on a vertical wall through homotopy-based scheme along with fractional calculus. Three cases were examined after considering (i) partial fractional differential equation (PFDE) by altering first-order derivative to fractional derivative in the interval (0, 1), (ii) PFDE by altering second-order derivative to fractional derivative in the interval (1, 2), and (iii) fully FDE by altering first-order derivative to fractional derivative in (0, 1) and second-order derivative to fractional derivative in (1, 2). Different physical quantities such as the velocity profile and volume flux were computed and analyzed. Validity of obtained results was checked by finding residuals. Moreover, consequence of different parameters on the velocity were also explored in fractional space.