Because it is a staple food, sustainable production of wheat is crucial for global food security. Arid and semi-arid regions are worst affected by climate change, which has resulted in poor productivity of different crops, including wheat. To this end, this study aimed to investigate the effect of foliage-applied thiourea on the growth, yield, and nutritional-quality-related traits of bread wheat. The treatments consisted of thiourea levels (control, 500, and 1000 mg L−1) factorally combined with two diverse wheat cultivars (Gandam-1 and Galaxy-2013) at different growth stages (tillering, booting, and heading) and was repeated over two years. The analysis of the data shows that thiourea treatments and the cultivars significantly (p ≤ 0.05) affected the growth, nutritional quality traits, and morphological traits, and the interaction of the two factors was also significant. Improvement in productivity and nutritional quality was observed from the application of thiourea in both cultivars. Galaxy-2013 performed best at 1000 mg L−1 thiourea application for both productivity- and nutritional-quality-related traits at the heading stage. In conclusion, exogenous application of thiourea improves the productivity and nutritional quality of wheat on sandy loam soils in semi-arid regions; however, for wider recommendations, more trials may be conducted across various agro-ecological regions.
Background: Outbreaks of small ruminant morbillivirus (SRMV) are regularly occurring in Pakistan despite vaccine availability. This study was designed to identify substitutions within the immunogenic structural and functional regions of the nucleocapsid, fusion, and hemagglutinin genes of SRMV and their comparison with vaccinal strains of Nigerian and Indian origin. Methods: Swabs and tissue samples were collected from diseased animals. RT-PCR was used to characterize selected genes encoded by viral RNA. The study’s N, F, and H protein sequences and vaccinal strains were analyzed for B and T cell epitope prediction using ABCpred, Bipred, and IEDB, respectively. Results: Significant substitutions were found on the C terminus of the nucleocapsid, within the fusion motif region of the fusion gene and in the immunoreactive region of the hemagglutinin gene. Conclusion: Our results emphasize the need for the development of effective vaccines that match the existing variants of SRMV strains circulating in Pakistan.
The yield potential of wheat crop is not achieved abundantly because of disease pressure. One of the most destructive of such diseases is stem rust (SR). SR caused by Puccinia graminis f. sp. tritici (Pgt), had been controlled successfully during three decades throughout the world with deployment of semi-dwarf resistant cultivars in the last half of previous century. During 1999 appearance and dispersion of stem rust race Ug-99 in Uganda (a virulent race against Sr31) created an alarming situation worldwide. Widespread germplasm was protected by gene Sr31 found susceptible to this terrible strain as the gene was protecting 80% wheat lines cultivated throughout planet. The emergence of the Ug99 race of stem rust in Africa and the Middle East together with the appearance of new strains in Europe catalyzed a main effort to recognize sources of stem rust resistance genes against new virulent strains and incorporate these genes into wheat lines. Scientific community addressed the dilemma in time and efforts did not go waste. Worldwide concern regarding the danger to global wheat production caused by Ug99 led to breeding wheat for durable resistance against disease and achieved considerably. This success is attributed to team work of experts and serves as an example for research workers in future. However, the continued emergence of stem rust variants that overcome new resistance genes, demands an amplified emphasis on pathogen evolution and virulence mechanisms. A major role for BGRI is to keep ‘the eye on the ball’ with regard to all these aspects. This article enables us to design strategy to tackle a situation which appears without alarm but in this case intellectuals coordinated each other and solution became possible. The same principle does not apply in plant pathology but in Human pathology and Veterinary pathology.
Stripe rust in wheat is the most problematic disease damaging the produce severely in wheat growing regions. In this paper findings in recent past related to yellow rust with reference to their application in the management of disease have been debated. In this regard various aspects of breeding for resistance were focused. Pathogen has adopted under the changed climatic scenario brings and disease is observed in the regions where it did not exist earlier. Rising temperatures has increased the irregularity and concentration of precipitation, causing the spread and severity of rust diseases. NB-LRR proteins are expected to play a substantial part in advanced agriculture where yield is the primary goal for screening. Biotechnology has revolutionized in biological sciences including Plant Pathology. Experts are studying the biochemical nature and the signals involved in, a plant's responses to pathogen attack and development of disease. Plant resistance genes are being engineered into plants to defend them from plant diseases.
Yellow (stripe) rust of wheat is responsible for a severe decrease in crop yield worldwide. Deployment of durable disease resistant cultivars is the best strategy being cost effective and safe. A comprehensive study was conducted to identify sources of durable resistance against stripe rust of wheat. Initially, surveillance of the crop was conducted to expose the status of the dilemma. The study discovered that disease is more prevalent in the region existing in Khyber Pakhtunkhwa (KPK) and the Northern Region of Punjab. Race identification of the pathogen under glasshouse conditions revealed high diversity and at least eleven races were designated. Prevalence of the race 70E0 was most dominating (39 %). Cultivation of multi-location trap nurseries yielded valuable information demonstrating disease-fighting genes where, at all locations, Yr5, Yr10, Yr15, and YrSp were effective. Seventy-two advanced lines collected from research institutions were screened in a glasshouse to categorize the test material based on their response to disease at the seedling stage. Test material comprising seventy-two advanced lines collected from different research institutions was screened in a glasshouse to categorize the test material on the ground of their response against disease at the seedling stage. Slow and fast rusters were categorized by studying susceptible and moderately susceptible seedlings in the field for two years. Genetic diversity in the host allows changes in the genetic organization to adapt to environmental changes. Coefficient parentage revealed the test material's restricted genetic base. In the pedigree of wheat advanced lines, Pastor, Kauz, Inqilab-91, Sokoll, Ae. Aquarosa (211), WBLL-1, Kukuna, and Millan were 60% out of a total of 72 parents. Findings of the present studies revealed persistent resistant genotypes with a broad genetic background are needed to feed a growing population.
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