A new spectrophotometric reagent, salicylaldehyde-orthoaminophenol (Sal-OAP) has been synthesized and characterized for the determination of selenium through novel reaction techniques. Also, a new highly selective, and sensitive spectrophotometric method for the nano-trace determination of selenium using salicylaldehyde-orthoaminophenol (Sal-OAP) has been developed. Sal-OAP undergoes reaction in a slightly acidic solution (0.0001-0.0002 M H2S04) with selenium (IV) to give an orange-red chelate, which has an absorption maximum at 379 nm. The reaction is instantaneous and absorbance remains stable for over 24 h. The average molar absorption co-efficient and Sandell’s sensitivity were found to be 6.4×105 L/mol.cm and 1.0 ng/cm2 of, respectively. Linear calibration graphs were obtained for 0.001-40.000 mg/Lof Se having detection limit of 0.1 µg/L and RSD 0-2 %. The stoichiometric composition of the chelate is 1:2 (Se:Sal-OAP). A large excess of over 60 cations, anions and some common complexing agents, such as chloride, azide, tartrate, EDTA, SCN¯etc., do not interfere in the determination. The developed method was successfully used in the determination of selenium in several Certified Reference Materials (Alloys, steels, human urine, bovine liver, drinking water, tea, milk, soil, and sediments) as well as in some environmental waters (Potable and polluted), biological fluids (Human blood, urine, hair, and milk), soil samples, food samples (Vegetables, rice, and wheat) and pharmaceutical samples (Tablet and syrup) and solutions containing both selenium (IV) and selenium (VI) as well as complex synthetic mixtures. The results of the proposed method for assessing biological, food and vegetables and soil samples were comparable with ICP-OES and AAS were found to be in excellent agreement. The method has high precision and accuracy (s = ±0.01 for 0.5 mg/L).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.