sectioning. Routine and special stained sections were studied under the light microscope and relevant findings were recorded. RESULTS: Data obtained from biochemical analyses and histomorphometry along with the histopathological features revealed that with increasing duration of hyperglycemia was associated with increased serum creatinine and reduction in serum total protein; increased mean percentage of darkly stained large sized pituicytes and notable thickening of perisinusoidal collagen. CONCLUSION: It is therefore concluded that long-standing hyperglycemia which is associated with increased occurrence of predominantly large and darkly stained pituicytes and remarkably increased deposition of perisinusoidal collagen appear to be the important contributing factors somehow responsible for the derangement of the function of the posterior pituitary in chronic diabetes.
Objectives: One of the common clinical observations regarding long-standing hyperglycemia is autonomic neuropathy, probably due to its unfavorable destructive effects on the neurons of the autonomic ganglia. Accordingly, the current study was aimed to analyze the effect of experimental hyperglycemia on parasympathetic pterygopalatine ganglion (PtG) and sympathetic coeliac ganglion (ClG) of albino rats.Methods: Thirty-six albino rats were divided into six groups (n=6, each) and were designated as control, two weeks, one month, two months, four months and six months groups. Diabetes was induced with a single dose of streptozotocin (STZ, 60 mg/kg, i.p.). Body weight and blood sugar were monitored at biweekly intervals. At the end of each experimental period, animals were euthanized by deep ether anesthesia and blood samples were collected by direct puncture of heart for biochemical analysis. Animals were perfused with Karnovsky fixative. After 48 hours, tissue samples were collected and processed for light microscopy.Results: Biochemical analysis of serum revealed increased serum creatinine and reduced serum total protein. Histopathology and histomorphometry of ganglia revealed that the progressively increasing duration of hyperglycemia was associated with decreased proportion of small-sized neurons, increased proportion of large-sized neurons, dark and dead neurons, and thickening of capsular and endoneurial collagen. Conclusion:The association of the long-standing hyperglycemia with increased neuronal death, altered proportion of neurons and deposition of collagen fibers in autonomic ganglia appear to be important contributing factors likely to be responsible for diabetic autonomic neuropathy.
Long standing hyperglycemia is generally associated with a decline in the chemical senses including smell due to its unfavorable destructive effects on the small sized neurons of the Olfactory bulb (OB). Accordingly, the current study was aimed to analyze the effect of experimental hyperglycemia on OB of albino rats. To detect a possible change on the olfactory bulb during hyperglycemia, 36 albino rats were divided into six groups of six rats each and were designated as control, two week, one month, two month, four month and six month respectively. Diabetes was induced with single dose of streptozotocin (STZ-60 mg/kg, IP). The inductions of diabetes were confirmed by measuring the blood glucose levels in the tail blood with a Glucometer. At the end of each experimental period animals were euthanized by deep ether anesthesia and blood samples were collected by direct puncture of heart for biochemical analysis. Animals were perfused with Karnovsky fixative. After two days tissue specimen were collected and processed for light microscopic studies. Biochemical analysis of serum revealed increased serum creatinine and reduced serum total protein. Histopathology and histomorphometry of OB revealed that the progressively increasing duration of hyperglycemia was associated with noticeable decreased diameter of OB glomeruli, decrease diameter of mitral cells and thickening of supporting connective tissue around the OB and blood vessels. It is concluded that the association of the long-standing hyperglycemia with frequent occurrence of hypercellular glomeruli and misplaced mitral cells, decreased diameter of OB glomeruli and mitral cells, subtle laminar disarray and added deposition of collagen fibers around OB and blood vessels appear to be important contributing factors responsible for the derangement of olfactory function in diabetics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.