Background: Diarrhea is one of the diseases that ranks in the top ten diseases in Depok City. Data from the Depok City Health Service (2020) states that diarrhea in toddlers is still above 5%. There are various factors (multifactor) related to diarrhea disease, one of which is nutritional status. Purposes: Our study aims to analyze the relationship between nutritional status and diarrhea in toddlers in the Pancoran Mas District Health Center. Methods: We used an observational analytic method with a cross-sectional approach on 396 toddlers aged 0 – 59 months. This research was conducted from October 2021 – March 2022. The type of data in this study is secondary data taken through medical records and analyzed using the chi-square test. Result: The prevalence of low-poor nutritional status was 38,9%, the prevalence of diarrhea was 21,2%, and the results of the chi-square analysis between diarrhea and nutritional status obtained p value = 0.334. Conclusion: Incidence of diarrhea in Depok City is still relatively large. There are many other factors that associated with the incidence of diarrhea such as personal hygiene, environmental hygiene and various other factors.
Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method.Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk.Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine’s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide.Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363.Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA).
Bandung memiliki ruang terbuka kota yang sudah banyak mengalami peremajaan dan didesain dengan menggunakan tema-tema tertentu. Sebagai sebuah fasilitas yang banyak digunakan oleh masyarakat, tentunya kelayakan fasilitas menjadi hal yang harus dipertimbangkan. Tujuan penelitian ini adalah untuk melakukan analisis terhadap kelayakan fasilitas ruang terbuka. Penelitian dilakukan melalui metoda pengamatan yang dilakukan pada Taman Saparua selama 2 bulan, dengan mengamati a) fasilitas utama, b) fasilitas penunjang, dan c) fasilitas pendukung. Kemudian dilakukan analisis menggunakan variabel penilaian terhadap kenyamanan, relaksasi, keterlibatan pasif, keterlibatan aktif dan penemuan. Hasil penelitian mendapatkan temuan berupa: penilaian yang tergolong dalam kateogri baik.
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