The interaction of Zn11 and Cd11 with bovine albumin: (A) the binding of Cd11 in the presence of 5.0 X 10 "4 ; (B) the binding of Zn11 in the presence of 5.0 X 10 M Cd11. (The broken curve shows the progressive displacement of Cd11 by Zn11). sites more reactive than imidazole: either the 0.7 sulfhydryl group of total albumin or a chelate site formed by an imidazole group and a neighboring carboxyl group. Klotz and co-workers12 have shown that the 375 µ absorption peak characteristic of the interaction of Cu11 with the sulfhydryl group of bovine albumin is depressed by ZnTI and Cd11, more so by Cd11 than by Zn11 and, (12) I.

Borax efficiently catalysed the synthesis of a series of spirooxindole and chromeno [2, 3-b]pyridine-3-carbonitrile derivatives in domino fashion via Knoevenagel condensation followed by Michael addition. This synthetic scheme is operationally simple, affording excellent yield and can be considered as an approach towards 'green chemistry'. In-silico docking studies of the synthesized molecules were carried to investigate the antitumor activity and to predict its binding affinity and orientation at the active site of human anaplastic lymphoma protein (Protein Data Bank (PDB): 2xp2). Molecular docking studies indicated that 2-amino-5'-chloro-7,7-dimethyl-2',5-dioxo-5,6,7,8-tetrahydrospiro[chromene-4, 3'-indoline]-3-carbonitrile (1 e) and 2 '-amino-5-chloro-2,5'-dioxo-5'H-spiro[indoline-3, 4'pyrano[3, 2-c]chromene]-3'-carbonitrile (2 b) appeared to show strong interactions at the receptor active site with predicted binding energy of À 8.47 kcal/mol and À 9.19 kcal/mol respectively. A detailed analysis of topology of the compounds 1 e and 2 b were also determined by X-ray crystallography and Hirshfeld surface analysis to get an insight of the packing and intermolecular interactions in their molecular structure.

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an over-all reaction for generation of radicals is as given below.

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