Karies gigi merupakan masalah kesehatan yang disebabkan Streptococcus mutans. Salah satu upaya mencegah karies gigi adalah melalui penggunaan mouthwash. Oleh karena mouthwash di pasaran tinggi alkohol sehingga meningkatkan risiko kanker mulut, maka diperlukan formulasi mouthwash berbahan dasar tanaman herbal. Tanaman herbal yang memiliki aktivitas antibakteri diantaranya teh hijau (Camellia sinensis) dan peppermint (Mentha piperita). Penelitian ini bertujuan untuk mengetahui efektivitas mouthwash ekstrak teh hijau dan peppermint sebagai antibakteri terhadap Streptococcus mutans. Aktivitas antibakteri ditentukan melalui pengukuran zona hambat pada uji difusi. Mouthwash dibuat dalam 5 formula yaitu F1, F2, F3, F4, F5 dengan konsentrasi 20%, 40%, 60%, 80%, dan 100%. Dibuat pula FA (mouthwash ekstrak teh hijau 20%) dan FB (mouthwash ekstrak peppermint 20%) sebagai pembanding. Hasil penelitian menunjukkan bahwa semakin tinggi konsentrasi ekstrak teh hijau dan peppermint maka semakin besar diameter zona hambat. Rata rata zona hambat F1 dan F2 8,34 dan 9,80 mm (daya hambat sedang). Rata rata zona hambat F3, F4 dan F5 masing masing 11,64 mm, 14,63 mm dan 15,91 mm (daya hambat kuat). Rata rata zona hambat FA dan FB 7,45 dan 6,20 mm (daya hambat sedang). Berdasarkan hasil tersebut disimpulkan bahwa mouthwash ekstrak Camellia sinensis dan Mentha piperita efektif sebagai antibakteri terhadap Streptococcus mutans.
Introduction: Awareness of Indonesian people in maintaining dental and oral health is low, proved by an increase in the percentage of dental and oral health problems by 2.7%. Caries is a dental and oral health problem that occurs in many children. The main cause of dental caries is Streptococcus mutans. To solve this problem, it is necessary to use herbal mouthwash made from a combination of Camellia sinensis and Mentha piperita extract as an antibacterial against Streptococcus mutans. Methods: Mouthwash is made through several processes namely plant determination, extraction, and mouthwash making. Minimal Inhibitory Concentration (MIC) is determined by diluted methods, Minimal Bactericidal Concentration (MBC) is determined by the agar streaking method, and colony tests are calculated using colony counters. Results: The result of plant determination showed the plants in this study were Camellia sinensis and Mentha piperita. At a concentration of 6.25%, no growth of bacteria in each repetition with the number of colonies 0 CFU / ml. While at a concentration of 3.125% found the average number of colonies 13 CFU / ml. Conclusions: Based on good MIC and MBC results, mouthwash containing Camellia sinensis and Mentha piperita has been shown to kill and inhibit the growth of Streptococcus mutans bacteria
Dengue Virus Infection (DVI) causes several clinical manifestations and requires varied diagnostic instruments. IgA anti-dengue as one of the diagnostic markers of DVI is suspected to have a shorter lifespan and greater sensitivity in detecting secondary infections compared to IgM anti-dengue. This study was conducted using 34 sera with positive RT-PCR or NS1 dengue virus. Samples were examined by a reverse flowimmunochromatographic method using AIM Dengue IgA Assure Rapid Test and will be analyzed its profile toward the day of fever, serotype, severity, platelet count, and type of infection. The overall sensitivity of IgA anti-dengue was 61.76% (n=34); in which IgA anti-dengue detected 14.29% primary and 66.67% secondary cases. IgA anti-dengue detected DEN1, DEN2, DEN3, and Mixed DEN1 – DEN3 virus serotype respectively 55.56%, 22.22%, 16.67%, and 5.56% (n=20). The day of fever was dominated by day-4 and day-5 respectively 28.57% (n=21). IgA anti-dengue was detected in DD, DHF grade I, II, and III 42,86%, 28.57%, 19.05%, and 9.52% (n=21) respectively. IgA anti-dengue detected in all levels of platelet count, it detected 60% in < 50,000 cell/mm3, 30% in 50,000 - 100.000 cell/mm3 and 10% in > 100,000 cell/mm3 platelet count sample (n=20). In conclusion, IgA anti-dengue showed a good performance, applicable as a diagnostic marker of DVI.
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