International collaboration via FTA cards may be helpful to improve diagnosis and management of patients with hematological malignancies in low-income and underdeveloped countries.
The lack of molecular diagnosis in the field of cancer in Iraq has motivated us to perform a genetic analysis of pediatric acute myelogenous leukemia (AML), including class I and II aberrations. Peripheral blood or bone marrow cells were collected from 134 AML children aged ≤15 years. Flinders Technology Associates (FTA) filter paper cards were used to transfer dried blood samples from five Iraqi hospitals to Japan. DNA sequencing was performed to identify class I mutations. Nested RT-PCR was used to detect class II aberrations, except that MLL rearrangement was detected according to long distance inverse-PCR. NPM1 and FMS-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) mutations were analyzed by GeneScan using DNA template. Among 134 Iraqi pediatric AML samples, the most prevalent FAB subtype was M2 (33.6 %) followed by M3 (17.9 %). Class I mutations: 20 (14.9 %), 8 (6.0 %), and 8 (6.0 %) patients had FLT3-ITD, FLT3-TKD, and KIT mutations, respectively. Class II mutations: 24 (17.9 %), 19 (14.2 %), and 9 (6.7 %) children had PML-RARA, RUNX1-RUNX1T1, and CBFB-MYH11 transcripts, respectively. MLL rearrangements were detected in 25 (18.7 %) patients. NPM1 mutation was detected in seven (5.2 %) cases. Collectively, approximately 30 % of AML children were proved to carry favorable prognostic genetic abnormalities, whereas approximately 10 % had high FLT3-ITD allelic burden and needed a special treatment plan including allogeneic hematopoietic stem cell transplantation. Acute promyelocytic leukemia (APL) was frequent among Iraqi pediatric AML. It is likely that molecular diagnosis using FTA cards in underdeveloped countries could guide doctors towards an appropriate treatment strategy.
Background and objective: : Leukemia is a significant public health and life-threatening problem for pediatric cancer patients. Adolescents leukemic may face long periods of treatment; may describe the irritability, fatigue, bone pain, mouth ulcer, alopecia, and loss of appetite. The aim of the study was to assess the common physical problems among leukemic adolescent patients undergoing chemotherapy, and identify the association between their socio-demographic characteristics with physical problem. Methods: A descriptive study was carried out in Nanakali Hospital for Blood Diseases / Erbil city from the period of /1 st Nov. /2010 to/ 1 st of Feb. /2011/. Eighty adolescent who are receiving chemotherapy in face to face interview, were selected regarding the study.
Results:The study shows that there were highly significant associations between sociodemographic characteristics with some of physical problems such as pain, fatigue, loss of appetite, and oral ulcer (mucositis).
Conclusion:The study shows that there were significant association between adolescent patient and some physical problems. The study recommends giving more support and attention by medical and nursing staff manage to reduce their physical problems.
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