The goal of this paper is to analyze the knowledge development on biomonitoring using comet assay on fishes. Were selected 154 papers in the ISI Web of Science and PubMed databases. The comet assay was used by 24.7% of the papers with the purpose to evaluate genotoxicity of polluted water. Comet assay is many times associated with other techniques, offering good results to increase efficiency in environmental assessment or to evaluate genotoxicity of pollutants individually.
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