99blood serum. With abnormal spinal fluid such as those obtained from syphililtic patients, higher inhibition titers were obtained, as to be expected. Saliva gave definite inhibition though only in low titer, in conformity with the known presence in this secretion of small amounts of .serum globulin. Only doubtful reactions were obtained with normal human semen and urine.Experiments were also conducted )to determine the ability of animal sera to inhibit the anti-human-globulin serum. As shown in the table, serum from horse, ox! and rablbit had no apparent inhibiting action. Rhesus serum weakened the clumping, even when highly diluted, but did not inhibit the reaction completely. This indicates the presence in rhesus slerum of a serum globulin chemically related [to, though qualitatively differenlt from the globulins in human serum.Summary and conclusions. 1. A new serological method od demonstrating human serum globulin has been described, based on its ability to inhibit the agglutinating action of anti-human-globulin serum for Rh-positive human red cells coated with Rho univalent anhibody.2. Using this technic, further evidence was obtained that the Rh antibody coating sensitized Rh-positive cells is a serum globulin.3 . With the new technic it is possible to demonstrate the presence in normal spinal fluid, and in saliva of small amounts of serum globulin, in proportion #to the known concentration in these fluids. Normal human semen and urine failed to inhibit the antiglobulin serum.4. Umbilical cord serum inhibited anti-globulin serum to the same titer as adult serum.5. Serum from horse, ox and rabbit did not inhibit tihe ant i-human-globulin serum, while rhesus monkey serum merely weakened its reactions.6. The new technic may find application not only in clinical medilcine, but also in forensic work for the examination of bilood stains, and in studies on biochemical evolution.
The general subject of lyophilization, with its specific applications, has been reviewed recently by Harris (1954). Although lyophilization has become a generally accepted method for long term preservation of stock cultures of viruses and bacteria it has not always been satisfactory for the preservation of Pasteurella pestis. This study was undertaken to determine some of the factors that affect viability of P. pestis during and after lyophilization.
A chemically defined culture medium which supported high viable cell yields of Pa8teurella pestis was described in a recent communication (Rockenmacher et al., 1952). While the amino 1 This work was supported by contracts between the University of California and the Office of Naval Research. ' The opinions and assertions contained in this report are the private ones of the writers and are not to be construed as official or reflecting the views of the Navy Department or the Naval Service at large (Article 1252, United States Navy Regulations, 1948).
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