Metabolomics
plays a pivotal role in systems biology, and NMR is
a central tool with high precision and exceptional resolution of chemical
information. Most NMR metabolomic studies are based on
1
H 1D spectroscopy, severely limited by peak overlap.
13
C NMR benefits from a larger signal dispersion but is barely used
in metabolomics due to ca. 6000-fold lower sensitivity. We introduce
a new approach, based on hyperpolarized
13
C NMR at natural
abundance, that circumvents this limitation. A new untargeted NMR-based
metabolomic workflow based on dissolution dynamic nuclear polarization
(d-DNP) for the first time enabled hyperpolarized natural abundance
13
C metabolomics. Statistical analysis of resulting hyperpolarized
13
C data distinguishes two groups of plant (tomato) extracts
and highlights biomarkers, in full agreement with previous results
on the same biological model. We also optimize parameters of the semiautomated
d-DNP system suitable for high-throughput studies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.