This study investigated photochemical investigation and extraction methods with polar and non polar solvent which could be used to compare and see the effectiveness of each on the chromosomes. The FTIR spectrum shows the presence of acetogenins in the extracts. Cytotoxic effects were studied using Allium cepa root tip cells. Eight onion bulbs were subjected to the treatment groups which were made using water, methanol, Ethyl acetate, acetone and hexane extracts of A. reticulata. Three different concentrations from each extraction solvent were used. The A. cepa root tip cells were subjected for 24 h to three different leaf extractions after left to grow in water. Concentrations of 0.1, 1, 10 and 50 mg/L were used. All studied concentrations of A. reticulata methanol extract showed significant (p ˂ 0.05) drop in the mean mitotic index when compared with control Overall decrease in MI was contributed to by all the stages of mitosis.
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