Intensive renal support in critically ill patients with acute kidney injury did not decrease mortality, improve recovery of kidney function, or reduce the rate of nonrenal organ failure as compared with less-intensive therapy involving a defined dose of intermittent hemodialysis three times per week and continuous renal-replacement therapy at 20 ml per kilogram per hour. (ClinicalTrials.gov number, NCT00076219.)

The molecular and cellular processes that lead to renal damage and to the heterogeneity of lupus nephritis (LN) are not well understood. We applied single-cell RNA sequencing (scRNA-seq) to renal biopsies from patients with LN and evaluated skin biopsies as a potential source of diagnostic and prognostic markers of renal disease. Type I interferon (IFN) response signatures in tubular cells and in keratinocytes distinguished patients with LN from healthy control subjects. Moreover, a high IFN response signature and fibrotic signature in tubular cells were each associated with failure to respond to treatment. Analysis of tubular cells from patients with proliferative, membranous, and mixed LN indicated pathways relevant to inflammation and fibrosis, which offer insight into their histological differences. In summary, we applied scRNA-seq to LN to deconstruct its heterogeneity and identify novel targets for personalized approaches to therapy.

Duplex Doppler sonography is a fundamental component of the complete ultrasonographic examination of the liver. Accurate interpretation of the spectral Doppler tracing from the hepatic veins is valuable, as it reflects important cardiac and hepatic physiology. Normally, there are four phases: A, S, V, and D; the S and D waves indicate flow in the antegrade direction toward the heart. In hepatic and cardiac disease, these normal waves may be absent, a finding indicative of flow in a nonphysiologic manner. In addition, transient patient factors such as phase of the respiratory cycle may influence the appearance of the spectral tracing. Familiarity with the normal and abnormal spectral Doppler waveforms from the hepatic veins and knowledge of their respective physiology and pathophysiology provide valuable insights. Systematic analysis of the direction, regularity, and phasicity of the spectral tracing and the ratio of the amplitudes of the S and D waves allows one to arrive at the correct differential diagnosis in most situations.

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