When comparing all the pigs treated with NACA vs. saline after hypoxia Fold Change of ASC in cortex was significantly reduced, p (Table 2).In hippocampus, cortex and Striatum Fold Change of IL-1b was elevated in all the hypoxia groups compared with the control group, p Conclusion NACA reduces the protein expression of Il-1beta and mRNA-expression of ASC in cortex after hypoxia. This may indicate that NACA has some neuroprotective abilities after perinatal asphyxia.Upcoming analyses of histopathology and injury markers will elucidate possible neuroprotective effects of NACA treatment following birth asphyxia. Background and aims Hypoxic-ischaemic encephalopathy (HIE) has been associated with long-term disabilities. Hypothermia is effective but does not provide complete neuroprotection, adjunctive therapies are necessary. Allopurinol has been proved as a good neuroprotector, but it has never been tested associated with hypothermia. The aim of the present study was to examine therapeutically effectiveness of dual therapy (hypothermia + allopurinol) versus hypothermia, in a neonatal rat model of HIE. Methods 120 Wistar pups at postnatal day 10 were used and divided into 5 groups: Sham-Operated, Hypoxic-ischaemic (HI) aggression, HI aggression + Allopurinol, HI aggression + Hypothermia, HI aggression + Hypothermia + Allopurinol.
PS-337At 25 day of life, spatial memory was assessed via water maze test. Finally, rats were anaesthetised and sacrified. In order to assess possible alterations in the hippocampal synaptic network, 3 specific synaptic proteins (PSD95, SNAP25, synaptophysin) were tested by Western Blot. Results There were differences in the learning outcomes among hypoxic, hypoxic + allopurinol, hypothermia, hypothermia + allopurinol and sham operated (p < 0,05). The worst group was the hypoxic one.Synaptophysin and SNAP25 levels were higher in controls and treatment groups compared with hypoxic untreated animals. However, the highest level of PSD95 corresponded to the hypoxic group. Conclusions Hypothermia and allopurinol seem to improve learning in HIE pups.Increased levels of presynaptic proteins in the treatment groups suggest that hypothermia and allopurinol improve synaptic plasticity compared with untreated group.PSD95 was also described in the literature as a suppressor of dendritic arbour development, so this could explain our results in the hypoxic group.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.