Bengal, India. One is Ramsagar Zone, which is famous for the fish culture and here the ponds are well maintained as well as the physico-chemical factors; the second one is Bankura zone, where some non-manicured ponds are selected and no special care was taken there. Thus, we measured out the relationship between the biological factors and also the environmental effects on those factors, specially the zooplanktons (evidently by comparison) of both the zonal water bodies. The physico-chemical parameters studied were the atmospheric temperature, water temperature, pH, alkalinity, dissolved oxygen, free carbon dioxide, bi-carbonate, organic carbon of soil, organic matter, primary productivity, phosphate-P, ammonium-N, nitrite-N, total hardness, chloride ion, electrical conductivity and the zooplankton as biological parameter. Materials and Methods:-Sampling program and procedure:-The total study was carried out consecutively for six months from September, 2015 to February, 2016 at twelve different perennial ponds located at two different zone of Bankura district (from Figure 3-14). The main goal of present study is to investigate the different physico-chemical characteristics; how effectively leads to change the biological characteristics specially zooplankton of this two zonal ponds. The water samples were collected from these twelve ponds. From Figure 2 (collected from satellite map) it is quite clear that the ponds of Ramsagar zone are closely distributed in surrounding Fishery firm and those ponds are used for fish culture. Thus the ponds are manicured to maintain a standardized comfortable environment for fish culture by local farmers. The first zone of our study is the Ramsagar zone, about 30 Km. away from Bankura town. This zone is very much popular for fishery and the ponds are well maintained; bathing and washing purposes are limited in those ponds. Pond R-1 (Hatibari 1) is large and situated far from locality beside a poultry farm house and the color of water is greenish. Pond R-2 (Hatibari 2) is just beside the R-1, same size to R-1, well manicured and water color is greenish. Pond R-3 (Sima bandh) is also well maintained, no weeds, regularly manicured, water color not so greenish. All three ponds are used for fish culture strictly and maintained by fish farmers. Pond R-4 (Hatibari 3) is not so large; it is used for regular purpose and seasonal fish culture. Pond R-5 (Hadgora pukur) is large, water color is greenish-red, well maintained and situated in the locality. Pond R-6 (Rakshit Pukur) is also large, water color greenish and situated in the locality. Both the ponds are used for local activity and some extend of seasonal fish culture. Beside the first zone, the second zone that is situated in Bankura town and the studied ponds are distributed throughout the southeastern part of Bankura town. Here the six sites (S-1, S-2, S-3, S-4, S-5 and S-6) of water collection were divided into three parts along with two nearer pond sites. S-1 and S-2 are the two ponds of Police line area, Bankura. The S-1 is within...