Isolated adrenocortical cells from 6 patients with a 'normal' zona fasciculata, 4 patients with a 'normal' zona glomerulosa, and tumour cells from 1 adrenocortical adenoma and 1 carcinoma were incubated with and without increasing concentrations of ACTH 1\p=n-\24 (10\m=-\13 m to 10\m=-\9 M) or Asp1-Ile5-angiotensin II (10\m=-\11 m to 10\ m=-\ 7 M). In 4/5 'normal' cases, cortisol was clearly stimulated by 10\m=-\13m ACTH. The maximum of the dose-response curve (5-fold stimulation) was reached at 10\m=-\10m ACTH. Angiotensin II (All) started to stimulate 'normal' cells at 10\m=-\11 m with a maximum (2-fold stimulation) at 10\m=-\9M. Aldosterone production by 'normal' cells was less markedly stimulated by ACTH and All, although the threshold doses for both peptides were similar to those of the cortisol response curves.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.