Since natural alternatives are needed in dentistry for the treatment of root canal, where the standard irrigant is NaOCl with significant toxicity, the aim of the study was to assess the antibacterial properties of non-chemical root-canal irrigants (aqueous extracts of oregano, thyme, lemongrass, melaleuca and clove essential oils) against Enterococcus faecalis. For this, aqueous extracts of each essential oil (AqEO) were prepared. A solution of sodium hypochlorite (NaOCl) was used as a positive standard against which the antimicrobial effects of AqEO could be reported. The root canals of seven teeth were inoculated with 20 µL of Enterococcus faecalis ATCC29212 inoculum and incubated overnight at 37 °C. All the teeth canals were instrumented and were irrigated with the corresponding AqEO, NaOCl and saline solution, then rinsed with saline. Bacteriological samples for each canal post-instrumentation were collected with sterile paper points which were inoculated on culture media. A second processing followed the same methodology but involved only irrigation and no instrumentation. Using instrumentation, thyme and clove completely inhibited Enterococcus faecalis growth. Without instrumentation, clove and oregano AqEOs completely reduced the bacterial load as seen in direct inoculation, but bacterial growth was observed in all the samples after enrichment, except for NaOCl. Nevertheless, the turbidity of the enrichment media was lower for the samples irrigated with AqEOs than for control. In conclusion, AqEOs of thyme, oregano and clove showed a promising antibacterial effect, especially when teeth instrumentation was performed.

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Dental sealants are an excellent means to prevent pits and fissure decay. Currently, there are multiple commercially available sealant materials. The purpose of this study was to assess the retention of glass carbomer fissure sealant and the incidence of secondary caries over a period of 24 months in comparison with a resin-based sealant. Materials and Methods: We included 32 children in the study, with ages between six and eight years and an average age of 6.8 years old. For each child, we sealed four permanent molars (totaling 128 teeth). The study group was divided into sub-groups. Sub-group A was represented by 64 first permanent molars which underwent dental sealing procedures with composite resin-based fissure sealant, Helioseal F™, and sub-group B was represented by 64 first permanent molars which underwent dental sealing procedures with glass carbomer cement, GCP Glass Seal™. The sealants were assessed clinically at 6, 12, 18, and 24 months. Results: The 6-month follow-up evaluation showed no statistically significant differences between the two materials neither regarding sealant retention nor new carious lesions formation (p > 0.05). At the 12-month recall, 57 molars had good retention (89.06%) from sub-group A and 44 molars (68.75%) from sub-group B; there was a statistically significant difference (p = 0.0187) between the two treatment choices only regarding material retention. At the last recall after 2 years, sub-group A had a higher number of molars with perfect sealing (47–73.43%) and 8 molars (12.5%) with new caries lesions and sub-group B had 23 (35.93%) molars with perfect sealing and 15 molars (23.44%) with new caries lesions; there was a statistically significant difference (p < 0.0001) between the two treatment choices only regarding material retention. Conclusions: The glass carbomer retention is very inferior to the resin-based material. The glass carbomer sealant was effective in preventing new caries development, comparable with the conventional resin-based sealant.

The aim of our study was to measure the cleaning efficiency of irrigating solutions used during endodontic treatment regarding smear layer removal from the root canal dentin walls. Ethylenediaminotetraacetic acid (EDTA) 17%, citric acid (CA) 10% and sodium hypochlorite (NaOCl) 2,5 % solutions were tested as final irrigating solutions. The study was conducted on extracted teeth, divided in four groups according to the irrigation protocol used. The specimens were analyzed by scanning electron microscopy and the amount of smear layer present at apical, middle and coronal level was recorded, based on a scoring system. Data were statistically analyzed using Kruskal-Wallis and Friedman test and the level of significance was set at p<0.05. In the coronal and middle segments of dental roots we noticed no statistically significant difference between EDTA and CA in smear layer removing capacity. Final irrigation with 17% EDTA proved to be more efficient than 10% CA and 2,5% NaOCl in smear layer removal at apical level of the root canal, with p<0.05 (p=0.042), which is an important area for disinfection in endodontic treatment.

Introduction:In the last 15 years, the most important revolution in dentistry has been the introduction and then the widespread adoption of the dental operating microscope. Theaimof this study was to evaluate the usage of the dental operating microscope among young specialized and general practitioner dentists in Tîrgu Mureș.Material and Methods:A 9-point questionnaire about dental microscope usage was distributed among endodontists, practitioners of other specialties, and general practitioners with less than 10 years of experience.Results:According to our results, the dental operating microscope is used mainly during root canal therapy by endodontists. Practitioners of other specialties (prosthetists, alveolar dental surgeons, and orthodontists) use the microscope in a small percentage during coronal restoration, prosthetics, or periodontal surgery. Most general practitioners are not interested in using magnification in their practice.Conclusions:Endodontists use the dental operating microscope when they face complicated cases and have access to it. Working under magnification is awakening the interest of doctors of other specialties also. Private practices should invest in acquiring a microscope, and young general practitioners should be motivated in using it during dental treatments.