Gas-phase electrophoresis of single-charged particles enables liposome characterization and finally the resulting vesicle encapsulation capacity determination.

Size, size distribution and molecular weight (MW) determination of nanoparticles and that are for example large polymers, are of great interest and pose an analytical challenge. In this context, nano electrospray gas‐phase electrophoretic mobility molecular analysis (nES GEMMA) is a valuable tool with growing impact. Separation of single‐charged analytes according to their electrophoretic mobility diameter (EMD) starting from single‐digit EMDs up to several hundred nm diameters is possible. In case of spherical analytes, the EMD corresponds to the dry nanoparticle size. Additionally, the instrument is capable of number‐based, single‐particle detection following the recommendation of the European Commission for nanoparticle characterization (2011/696/EU). In case an EMD/MW correlation for a particular compound class (based on availability of well‐defined standards) exists, a nanoparticle's MW can be determined from its EMD. In the present study, we focused on nES GEMMA of linear and branched, water‐soluble polysaccharides forming nanoparticles and were able to obtain spectra for both analyte classes regarding single‐charged species. Based on EMDs for corresponding analytes, an excellent EMD/MW correlation could be obtained in case of the branched natural polymer (dextran). This enables the determination of dextran MWs from nES GEMMA spectra despite high analyte polydispersity and in a size/MW range, where classical mass spectrometry is limited. EMD/MW correlations based on linear (pullulans, oat‐ß‐glucans) polymers were significantly different, possibly indicating challenges in the exact MW determination of these compounds by, for example, chromatographic and light scattering means. Despite these observations, nES GEMMA of linear, monosaccharide‐based polymers enabled the determination of size and size‐distribution of such dry bionanoparticles.